快速可靠的基于单核苷酸多态性的布鲁氏菌活疫苗株与田间分离株的区分。
Rapid and reliable single nucleotide polymorphism-based differentiation of Brucella live vaccine strains from field strains.
机构信息
Department of Statutory and Exotic Bacterial Disease, Veterinary Laboratories Agency, Woodham Lane, New Haw, United Kingdom.
出版信息
J Clin Microbiol. 2010 Apr;48(4):1461-4. doi: 10.1128/JCM.02193-09. Epub 2010 Feb 24.
Abstract
The reliable differentiation of live Brucella vaccine strains from field isolates is an important element in brucellosis control programs. We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrinsic part of, a previously described assay identifying Brucella isolates to the species level (K. K. Gopaul, C. J. Smith, M. S. Koylass, and A. M. Whatmore, BMC Microbiol. 8:86), giving a comprehensive molecular typing platform.
摘要
可靠地区分活疫苗株和野外分离株是布氏杆菌病控制计划的一个重要元素。我们描述了一种新的基于单核苷酸多态性（SNP）的分型平台的设计、验证和实施，该平台提供了一种快速、可靠和强大的工具，与现有分子检测相比，提高了诊断准确性。此外，所描述的检测方法旨在补充并可以作为先前描述的鉴定布鲁氏菌种水平的分离物的检测方法的内在部分（K. K. Gopaul、C. J. Smith、M. S. Koylass 和 A. M. Whatmore，BMC Microbiol. 8:86），从而提供了一个全面的分子分型平台。
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Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens.当前对布鲁氏菌属（一个不断扩大的人畜共患病病原体属）遗传多样性的认识。
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