Sagar Institute of Research and Technology-Pharmacy, Bhopal, India.
Nat Prod Res. 2010 Apr;24(6):534-41. doi: 10.1080/14786410802531782.
The aim of the present study was to assess the wound-healing activity of ethanolic extracts of Acorus calamus leaves. A wound was induced by an excision- and incision-based wound model in rats of either sex. The mature green leaves of A. calamus were collected and authenticated. Extractions of dried leaves were carried out with 80% ethanol in a soxhlet apparatus. For wound-healing activity, the extracts were applied topically once daily in conc. of 40% w/w and 20% w/w in the form of ointment and compared with a standard drug (povidion-iodine). The healing of the wound was assessed by the rate of wound closure, period of epithelialisation, tensile strength and weight of the granulation tissue, hydroxyproline content and histopathology of the granulation tissue. The ethanolic extract of A. calamus promoted wound-healing activity significantly in both the wound models studied. The histological study of the granulation tissue with 20% A. calamus extract ointment-treated animals showed a larger number of inflammatory cells and lesser collagen when compared with the 40% A. calamus extract ointment-treated animals. However, this was better than the control group of animals. Enhanced wound contraction, decreased epithelialisation time, increased hydroxyproline content and histological characteristics suggest that A. calamus extract may have therapeutic benefits in wound healing.
本研究旨在评估菖蒲叶乙醇提取物的伤口愈合活性。通过在雌雄大鼠上进行基于切除和切开的伤口模型来诱导伤口。采集成熟的菖蒲绿色叶片并进行鉴定。用索氏提取器用 80%乙醇对干叶进行提取。对于伤口愈合活性，将提取物以 40%w/w 和 20%w/w 的浓度以软膏的形式每天局部施用一次，并与标准药物（聚维酮碘）进行比较。通过伤口闭合率、上皮化时间、拉伸强度和肉芽组织重量、羟脯氨酸含量以及肉芽组织的组织病理学来评估伤口的愈合。菖蒲的乙醇提取物在两种研究的伤口模型中均显著促进了伤口愈合活性。用 20%菖蒲叶提取物软膏处理的动物的肉芽组织的组织学研究表明，与用 40%菖蒲叶提取物软膏处理的动物相比，有更多的炎症细胞和较少的胶原。然而，这比动物的对照组要好。增强的伤口收缩、减少的上皮化时间、增加的羟脯氨酸含量和组织学特征表明，菖蒲提取物可能对伤口愈合具有治疗益处。
