菖蒲对大鼠全层皮肤伤口胶原蛋白成熟的功效。
Efficacy of Acorus calamus on collagen maturation on full thickness cutaneous wounds in rats.
作者信息
Ponrasu Thangavel, Madhukumar Karuppanan Natarajan, Ganeshkumar Moorthy, Iyappan Kuttalam, Sangeethapriya Vilvanathan, Gayathri Vinaya Subramani, Suguna Lonchin
机构信息
Department of Biochemistry, Council of Scientific and Industrial Research-Central Leather Research Institute, Tamil Nadu, India.
Shirmpex Biotech Services, SSN College of Engineering, Kalavakkam, Chennai, Tamil Nadu, India.
出版信息
Pharmacogn Mag. 2014 Apr;10(Suppl 2):S299-305. doi: 10.4103/0973-1296.133283.
BACKGROUND
The rhizomes of Acorus calamus and their essential oil are widely used in the flavoring industry and production of alcoholic beverages in Europe. Recent reports have confirmed the presence of several pharmacological components in the rhizomes of A. calamus.
OBJECTIVE
The objective of this study was to find out the efficacy of topical administration of ethanolic extract of A. calamus on dermal wound healing in rats. Wound healing is a natural process occurring in living organisms, which results in a complete or partial remodeling of injured tissue and ultimately progresses to the formation of a fibrous scar. Several natural products have been reported to augment the wound healing process.
MATERIALS AND METHODS
An ethanolic extract of A. calamus was prepared and its wound-healing efficacy was studied. An excision wound was made on the back of the rat and 200 μL (40 mg/kg body weight) of the A. calamus extract was applied topically once daily for the treated wounds. The control wounds were treated with 200 μL of phosphate buffered saline.
RESULTS
The granulation tissues formed were removed at 4, 8 and 12 days and biochemical parameters such as deoxyribonucleic acid, total protein, total collagen, hexosamine and uronic acids were measured. The amount of type I/III collagen formed in control and treated wound tissues was evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The epithelialization time, tensile strength and histological examination of the wounds were also studied. Biochemical analyses of the granulation tissues revealed a significant increase in collagen, hexosamine and uronic acid when compared with the control. The tensile strength of extract treated wounds was found to increase by 112%. A significant reduction in lipid peroxide levels suggested that A. calamus possesses antioxidant components.
CONCLUSIONS
The results strongly confirm the beneficial effects of A. calamus in augmenting the wound healing process.
背景
菖蒲的根茎及其精油在欧洲的香料工业和酒精饮料生产中被广泛使用。最近的报道证实了菖蒲根茎中存在多种药理成分。
目的
本研究的目的是探究菖蒲乙醇提取物局部给药对大鼠皮肤伤口愈合的疗效。伤口愈合是生物体中发生的一个自然过程，会导致受损组织的完全或部分重塑，并最终发展为形成纤维瘢痕。据报道，几种天然产物可促进伤口愈合过程。
材料与方法
制备菖蒲乙醇提取物并研究其伤口愈合疗效。在大鼠背部制造切除伤口，对于治疗组伤口，每天局部涂抹200 μL（40 mg/kg体重）的菖蒲提取物。对照组伤口用200 μL磷酸盐缓冲盐水处理。
结果
在第4、8和12天移除形成的肉芽组织，并测量生化参数，如脱氧核糖核酸、总蛋白、总胶原蛋白、氨基葡萄糖和糖醛酸。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳评估对照和治疗伤口组织中形成的I/III型胶原蛋白的量。还研究了伤口的上皮化时间、抗张强度和组织学检查。肉芽组织的生化分析显示，与对照组相比，胶原蛋白、氨基葡萄糖和糖醛酸显著增加。发现提取物处理伤口的抗张强度增加了112%。脂质过氧化物水平的显著降低表明菖蒲具有抗氧化成分。
结论
结果有力地证实了菖蒲在促进伤口愈合过程中的有益作用。
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