Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
J Ethnopharmacol. 2012 Aug 1;142(3):714-22. doi: 10.1016/j.jep.2012.05.048. Epub 2012 Jun 7.
Pedilanthus tithymaloides leaves are widely used in Indian medicine to heal wounds, burn, mouth ulcers. However, systematic evaluation of these activities is lacking. Thus, the present study aimed to assesses the wound healing activity of Pedilanthus leaves and its isolated constituents in topical ointment formulation.
Bioassay-guided chromatographic fractionation of the methanol extract of leaves resulted in the isolation of 2-(3,4-dihydroxy-phenyl)-5,7-dihydroxy-chromen-4-one and 1, 2-tetradecanediol, 1-(hydrogen sulfate), sodium salt. The ointment formulation of methanol extract (2.5%, 5% w/w) and isolated compounds (0.25% w/w) was prepared and evaluated on excision, incision and dead space wound models in rats. The effects of formulations on wound healing were assessed by the rate of wound closure, period of epithelialization, tensile strength, granulation tissue weight, hydroxyproline content and histopathology.
Significant wound healing activity was observed with methanol extract and isolated constituents. Topical application of isolated compound ointments caused faster epithelialization, significant wound contraction (95.41%), and better tensile strength (565.33 g) on 16 post-wounding day, while 5% extract showed wound epithelialization with 95.55% contraction on 18th post-wounding day, better than the control group (76.39% on 22 day). The tensile strength of incision wound was significantly increased in extract and compound treated animals. Moreover, in dead space model the extract significantly increased granuloma tissue weight, tensile strength and hydroxyproline content. The tissue histology of ointment treated groups showed complete epithelialization with increased collagenation, compared to the povidone-iodine group.
The results validated the traditional use of Pedilanthus tithymaloides for cutaneous wound management.
猪殃殃叶在印度医学中被广泛用于治疗伤口、烧伤和口腔溃疡。然而，这些用途的系统评价却很缺乏。因此，本研究旨在评估猪殃殃叶及其分离成分在局部软膏制剂中的伤口愈合活性。
生物活性导向的叶甲醇提取物的色谱分离导致了 2-(3,4-二羟基-苯基)-5,7-二羟基色原酮-4-酮和 1,2-十四烷二醇，1-(硫酸氢盐)，钠盐的分离。甲醇提取物（2.5%，5%w/w）和分离化合物（0.25%w/w）的软膏制剂被制备并在大鼠的切除、切口和死腔伤口模型中进行评估。通过伤口闭合率、上皮化时间、拉伸强度、肉芽组织重量、羟脯氨酸含量和组织病理学来评估制剂对伤口愈合的影响。
甲醇提取物和分离成分均表现出显著的伤口愈合活性。局部应用分离化合物软膏可导致更快的上皮化、显著的伤口收缩（95.41%）和更好的拉伸强度（565.33g），在 16 天的创伤后，而 5%的提取物在 18 天的创伤后显示出 95.55%的伤口上皮化，优于对照组（22 天的 76.39%）。在提取物和化合物处理的动物中，切口伤口的拉伸强度显著增加。此外，在死腔模型中，提取物显著增加了肉芽组织重量、拉伸强度和羟脯氨酸含量。软膏处理组的组织学显示出完全的上皮化，与聚维酮碘组相比，胶原形成增加。
这些结果验证了猪殃殃用于皮肤伤口管理的传统用途。
