Liu Siqi, Zhang Xiaoxue, Zhang Guorui, Zhao Jinmei, Zhang Xiaoqing
Institute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, China.
College of Life Sciences, Northwest A &F University, Yangling, Shan Xi, China.
Front Plant Sci. 2025 May 14;16:1598628. doi: 10.3389/fpls.2025.1598628. eCollection 2025.
Freezing injury in winter is a major abiotic stress that significantly limits plant growth and survival. While nitrogen and phosphorus fertilizers have been demonstrated to alleviate the impact of freezing injury in various plant species, their role of fertilizers in the cold tolerance of spp. is still unknown.
This study investigated the effects of fertilizers on the cold resistance of by comprehensively analyzing the physiological and biochemical indices, transcriptome, and metabolome of the under applications of 150 kg nitrogen ha-1 (N) and 90 kg phosphorus ha-1 (P), using "no fertilizer" (CK) as the control.
The results showed that applying nitrogen and phosphorus fertilizers reduced the malondialdehyde concentration and had much higher superoxide dismutase activity and soluble sugar and proline concentrations. Transcriptomics and metabolomics analysis revealed that applying nitrogen and phosphorus fertilizers tended to involve several critical regulatory pathways in the biosynthesis of secondary metabolites, flavonoid biosynthesis, and phenylpropanoid biosynthesis pathways. Concretely speaking, these fertilizers can affect the biosynthesis of naringenin, pinobanksin 3-acetate, galangin, and p-Coumaroyl shikimic acid and the expression of related genes to regulate the cold tolerance of . Moreover, through using weighted correlation network analysis (WGCNA), 4210 genes in response to nitrogen fertilizer and 5975 genes in response to phosphorus fertilizer, positively correlating with key metabolites, were identified. Several genes encoding enzymes including glucan endo-1,3-beta-glucosidase, pectinesterase, trehalase, hydroquinone glucosyltransferase, monodehydroascorbate reductase, tyrosine aminotransferase, and peroxidase were verified to be hub genes involved in the cold-stress response of .
Overall, these findings have laid a theoretical foundation for the highly efficient utilization of nitrogen and phosphorus in and provide novel insights into the regulatory network of in response to cold-temperature stress.
冬季冻害是一种主要的非生物胁迫，严重限制了植物的生长和存活。虽然氮肥和磷肥已被证明可减轻多种植物物种的冻害影响，但它们在[物种名称]抗寒性中的作用仍不清楚。
本研究通过综合分析在施用150千克氮/公顷（N）和90千克磷/公顷（P）条件下[物种名称]的生理生化指标、转录组和代谢组，以“不施肥”（CK）作为对照，研究了肥料对[物种名称]抗寒性的影响。
结果表明，施用氮肥和磷肥降低了丙二醛浓度，超氧化物歧化酶活性以及可溶性糖和脯氨酸浓度更高。转录组学和代谢组学分析表明，施用氮肥和磷肥倾向于涉及次生代谢物生物合成、类黄酮生物合成和苯丙烷生物合成途径中的几个关键调控途径。具体而言，这些肥料可影响柚皮素、3-乙酸松脂醇、高良姜素和对香豆酰莽草酸的生物合成以及相关基因的表达，以调节[物种名称]的抗寒性。此外，通过使用加权基因共表达网络分析（WGCNA），鉴定出4210个响应氮肥的基因和5975个响应磷肥的基因，它们与关键代谢物呈正相关。几个编码包括内切-1,3-β-葡聚糖酶、果胶酯酶、海藻糖酶、对苯二酚葡糖基转移酶、单脱氢抗坏血酸还原酶、酪氨酸转氨酶和过氧化物酶在内的酶的基因被证实是参与[物种名称]冷胁迫响应的枢纽基因。
总体而言，这些发现为[物种名称]中氮和磷的高效利用奠定了理论基础，并为[物种名称]响应低温胁迫的调控网络提供了新的见解。
