Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing, PR China.
J Sep Sci. 2010 Apr;33(8):1004-9. doi: 10.1002/jssc.200900690.
A simple and rapid method for the simultaneous determination of seven isoquinoline alkaloids, protopine, chelidonine, coptisine, stylopine, sanguinarine, berberine, and chelerythrine, in Chelidonium majus L. (Ch. majus) samples by ultra-performance LC method with photodiode array detection is described. The baseline separation of these compounds was performed with (A) acetonitrile-(B) ammonium acetate (10 mM, adjusted to pH 3.0 with acetic acid) as the mobile phase using a C18 RP column (2.1x100 mm, 1.7 microm). Optimized conditions resulted in excellent peak shapes. The seven alkaloids were completely separated within 20 min. Good linear behaviors (r > or = 0.9992) over the investigated concentration ranges were observed for all the analytes. Validation proved the repeatability of the method was good and recovery was satisfactory. The validated method was successfully applied for 20 batches of Ch. majus. These results demonstrated that the ultra-performance LC photodiode array method proposed was very useful in the analysis and quality control of Ch. majus.
一种简单快速的超高效液相色谱法，同时测定白屈菜样品中 7 种异喹啉生物碱，原阿片碱、白屈菜碱、黄连碱、斯图尔碱、血根碱、小檗碱和白屈菜红碱，用光电二极管阵列检测。这些化合物的基线分离是用（A）乙腈-（B）10 mM 乙酸铵（用乙酸调至 pH 3.0）作为流动相，在 C18 RP 柱（2.1x100mm，1.7 微米）上进行的。优化的条件得到了极好的峰形。7 种生物碱在 20 分钟内完全分离。所有分析物在考察的浓度范围内均表现出良好的线性关系（r >或= 0.9992）。验证证明该方法的重复性良好，回收率令人满意。该验证方法成功应用于 20 批白屈菜。这些结果表明，所提出的超高效液相色谱光电二极管阵列法在白屈菜的分析和质量控制中非常有用。
