Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Int J Mol Sci. 2023 Mar 28;24(7):6360. doi: 10.3390/ijms24076360.
The search for new substances with cytotoxic activity against various cancer cells, especially cells that are very resistant to currently used chemotherapeutic agents, such as melanoma cells, is a very important scientific aspect. We investigated the cytotoxic effect of , and extracts obtained from different parts of these plants collected at various vegetation stages on FaDu, SCC-25, MCF-7, and MDA-MB-231 cancer cells. Almost all the tested extracts showed higher cytotoxicity against these cancer cells than the anticancer drug etoposide. The highest cytotoxicity against the FaDu, SCC-25, MCF-7 and MDA-MB-231 cancer cell lines was obtained for the extract collected before flowering. The cytotoxicity of extracts obtained from different parts of collected at various vegetation stages was also evaluated on melanoma cells (A375, G361 and SK-MEL-3). The highest cytotoxic activity against melanoma A375 cells was observed for the root extract, with an IC50 of 12.65 μg/mL. The same extract was the most cytotoxic against SK-MEL-3 cells (IC50 = 1.93 μg/mL), while the highest cytotoxic activity against G361 cells was observed after exposure to the extract obtained from the herb of the plant. The cytotoxic activity of extracts against melanoma cells was compared with the cytotoxicity of the following anticancer drugs: etoposide, cisplatin and hydroxyurea. In most cases, the IC50 values obtained for the anticancer drugs were higher than those obtained for the extracts. The most cytotoxic extract obtained from the root of was selected for in vivo cytotoxic activity investigations using a Danio rerio larvae xenograft model. The model was applied for the first time in the in vivo investigations of the extract's anticancer potential. The application of larvae xenografts in cancer research is advantageous because of the transparency and ease of compound administration, the small size and the short duration and low cost of the experiments. The results obtained in the xenograft model confirmed the great effect of the investigated extract on the number of cancer cells in a living organism. Our investigations show that the investigated plant extracts exhibit very high cytotoxic activity and can be recommended for further experiments in order to additionally confirm their potential use in the treatment of various human cancers.
寻找具有细胞毒性的新物质，特别是针对各种癌细胞，特别是对目前使用的化疗药物非常耐药的癌细胞，如黑色素瘤细胞，是一个非常重要的科学方面。我们研究了从这些植物的不同部位在不同生长阶段采集的提取物对 FaDu、SCC-25、MCF-7 和 MDA-MB-231 癌细胞的细胞毒性作用。几乎所有测试的提取物对这些癌细胞的细胞毒性都高于抗癌药物依托泊苷。在开花前采集的提取物对 FaDu、SCC-25、MCF-7 和 MDA-MB-231 癌细胞系的细胞毒性最高。还评估了从不同生长阶段采集的不同部位的提取物对黑色素瘤细胞（A375、G361 和 SK-MEL-3）的细胞毒性。对黑色素瘤 A375 细胞的最高细胞毒性活性观察到根提取物，IC50 为 12.65μg/mL。相同的提取物对 SK-MEL-3 细胞的细胞毒性最高（IC50=1.93μg/mL），而对 G361 细胞的最高细胞毒性活性则是在暴露于植物草药提取物后观察到的。将 提取物的细胞毒性与以下抗癌药物的细胞毒性进行了比较：依托泊苷、顺铂和羟基脲。在大多数情况下，获得的抗癌药物的 IC50 值高于获得的 提取物的 IC50 值。从 根中获得的最具细胞毒性的提取物被选为使用斑马鱼幼虫异种移植模型进行体内细胞毒性活性研究。该模型首次应用于提取物抗癌潜力的体内研究。应用斑马鱼幼虫异种移植进行癌症研究是有利的，因为化合物给药方便、体积小、实验时间短、成本低。在异种移植模型中获得的结果证实了所研究的提取物对活生物体中癌细胞数量的巨大影响。我们的研究表明，所研究的植物提取物具有非常高的细胞毒性活性，可以推荐进行进一步的实验，以进一步证实它们在治疗各种人类癌症中的潜在用途。
Phytomedicine. 2015-12-15
Drug Des Devel Ther. 2025-1-11
Materials (Basel). 2024-8-24
Int J Mol Sci. 2021-2-6
Molecules. 2020-11-10
Bull Exp Biol Med. 2020-8
Oxid Med Cell Longev. 2019-12-14
Zotero 插件