[反相高效液相色谱法测定白屈菜中白屈菜红碱的含量]
[Determination of chelerythrine in Chelidonium majus by RP-HPLC].
作者信息
Sun Nan, Yu Liming
机构信息
Dalian Institute for Drug Control, Dalian 116021, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2918-9.
OBJECTIVE
To develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.
METHOD
Chelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (25:75) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.
RESULT
The linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.
CONCLUSION
The method is accurate, sensitive and reliable.
目的
建立一种测定白屈菜中白屈菜红碱含量的高效液相色谱法。
方法
用甲醇从粉碎后的白屈菜细粉中提取白屈菜红碱，采用高效液相色谱法测定。流动相为乙腈 - 1%三乙胺（25∶75）（用磷酸调pH值至3），检测波长设定为268 nm。
结果
校正曲线的线性范围为0.051 6 - 0.516 0 μg（r = 1.000）。平均回收率（n = 6）为103.0%，相对标准偏差为1.2%。样品溶液中的白屈菜红碱在8小时内稳定，在3种不同色谱柱上的耐用性良好。
结论
该方法准确、灵敏、可靠。
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