Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou 215006, People's Republic of China.
Cell Biol Int. 2010 Apr 14;34(6):573-7. doi: 10.1042/CBI20090248.
We explored the effects on brain oedema and neurological functional recovery after transplantation of hAECs (human amniotic epithelial cells) into the lateral ventricle of rats with ICH (intracerebral haemorrhage). hAECs were isolated from human term placenta and seeded for primary culture. We delivered hAECs labelled with Hoechst33258 and transfected with EGFP (enhanced green fluorescent protein) gene using lentiviral vectors into ICH rat models. The behaviour of the animals and brain oedema were evaluated after 28 days, and brain sections were made for morphological and immunohistochemical analyses with fluorescence microscopy. Our results were as follows. Transplanted hAECs were observed along the lateral wall and survived for at least 4 weeks. Some of the cells were stained with human specific antibody to vimentin and nestin. Around the injury site, activated microglia stained with OX42 were reduced. The water content of ICH rats decreased in the treatment group. The behaviour test scores were improved in the treatment group compared with those in the control groups. In conclusion, hAECs cannot only survive in the lateral ventricle of ICH rats after transplantation, but also express vimentin and nestin. hAEC transplantation reduced brain oedema and improved the motor deficits of ICH rats.
我们研究了将人羊膜上皮细胞（hAECs）移植到脑出血（ICH）大鼠侧脑室后对脑水肿和神经功能恢复的影响。hAECs 从人足月胎盘分离出来并进行原代培养。我们使用慢病毒载体将标记有 Hoechst33258 的 hAECs 和转染有 EGFP（增强型绿色荧光蛋白）基因的 hAECs 递送到 ICH 大鼠模型中。在 28 天后评估动物的行为和脑水肿，并制作脑切片进行形态学和免疫组织化学分析，用荧光显微镜观察。我们的结果如下。移植的 hAECs 沿侧壁观察到并至少存活了 4 周。一些细胞被波形蛋白和巢蛋白的人特异性抗体染色。在损伤部位周围，用 OX42 染色的活化小胶质细胞减少。治疗组 ICH 大鼠的含水量降低。与对照组相比，治疗组的行为测试评分有所提高。总之，hAECs 不仅可以在移植到 ICH 大鼠的侧脑室中存活，而且还可以表达波形蛋白和巢蛋白。hAEC 移植可减少脑水肿并改善 ICH 大鼠的运动功能障碍。
