Drago Lorenzo, Nicola Lucia, Iemoli Enrico, Banfi Giuseppe, De Vecchi Elena
Laboratory of Microbiology, Department of Preclinical Sciences LITA Vialba, University of Milan, Milan, Italy.
BMC Res Notes. 2010 Feb 25;3:44. doi: 10.1186/1756-0500-3-44.
Oral administration of probiotics is known to modulate cytokines profile not only locally, but also systemically. Four strains of Lactobacillus salivarius, LDR0723, BNL1059, RGS1746 and CRL1528, were evaluated for their ability to modulate release of pro- and anti-inflammatory cytokines.
Strains were assessed for effects on production of Interleukin-12 (IL-12), Interferon-gamma (IFN-gamma), Interleukin-4 (IL-4) and Interleukin-5 (IL-5) by incubating bacterial suspensions with THP-1 macrophage like cells. Cytokines were determined by means of specific quantitative enzyme-linked immunosorbent assays.LDR0723 and CRL1528 led to a sustained increment in production of IL-12 and IFN-gamma and to a decrease in release of IL-4 and IL-5, while BNL1059 and RGS1746 favoured Th2 response, leading to a decrease in Th1/Th2 ratio with respect to unstimulated cells.
In conclusion, capability of L. salivarius to modulate immune response was strictly strain dependent and strains of the same species might have opposite effects. Therefore, a careful evaluation of anti-inflammatory properties of lactobacilli should be performed on single strain, before any consideration on potential probiotic use.
已知口服益生菌不仅能在局部调节细胞因子谱，还能在全身发挥作用。对唾液乳杆菌的四株菌株LDR0723、BNL1059、RGS1746和CRL1528调节促炎和抗炎细胞因子释放的能力进行了评估。
通过将细菌悬液与THP-1巨噬细胞样细胞孵育，评估菌株对白细胞介素-12（IL-12）、干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）和白细胞介素-5（IL-5）产生的影响。通过特定的定量酶联免疫吸附测定法测定细胞因子。LDR0723和CRL1528导致IL-12和IFN-γ的产生持续增加，IL-4和IL-5的释放减少，而BNL1059和RGS1746则有利于Th2反应，相对于未刺激的细胞，导致Th1/Th2比值降低。
总之，唾液乳杆菌调节免疫反应的能力严格取决于菌株，同一物种的菌株可能有相反的作用。因此，在考虑潜在的益生菌用途之前，应在单一菌株上仔细评估乳杆菌的抗炎特性。
