Park Mi-Sung, Song Nho-Eul, Baik Sang-Ho, Pae Hyun-Ock, Park Seong Hoon
Institute for Metabolic Disease, Wonkwang University, Iksan, Jeollabuk 570-749, Republic of Korea.
Department of Food Science and Human Nutrition, and Fermented Food Research Center, Chonbuk National University, Jeonju, Jeollabuk 561-756, Republic of Korea.
Exp Ther Med. 2017 Jul;14(1):635-641. doi: 10.3892/etm.2017.4536. Epub 2017 Jun 1.
Certain strains of lactobacilli have been reported to exert favorable effects on atopic dermatitis (AD). , a traditional Korean food, is a salted fermented seafood known to harbor many lactic acid bacteria. In the present study, two novel lactobacillus strains were isolated from , and their anti-AD effects were investigated. Lactobacilli isolated from were identified, according to conjugated linoleic acid-producing activity, as (JBCC105645 and JBCC105683). AD-like skin lesions were induced in BALB/c mice using dinitrofluorobenzene (DNFB). Ear swelling, histological analysis and serum immunoglobulin E (IgE) levels in mice were evaluated to investigate the anti-AD effects of lactobacilli. Cytokine production of cluster of differentiation (CD)4 T cells, and interleukin (IL)-12 production of macrophages were also evaluated to establish a putative mechanism of the action of lactobacilli. Administration of JBCC105645 or JBCC105683 suppressed ear swelling and serum IgE levels in DNFB-treated mice (P<0.05). Notably, JBCC105645 was more effective than JBCC105683 (P<0.05). Treatment with the lactobacilli also induced a significant decrease in IL-4 production with concomitant increase in interferon (IFN)-γ production in DNFB-exposed CD4 T cells, and an increase in IL-12 production in macrophages (P<0.05). Taken together, the lactobacilli isolated from may suppress the development of AD-like skin inflammation in mice by modulating IL-4 and IFN-γ production in CD4 T cells, presumably via enhancing IL-12 production by macrophages.
据报道，某些乳酸杆菌菌株对特应性皮炎（AD）具有有益作用。泡菜是一种传统的韩国食品，是一种已知含有许多乳酸菌的腌制发酵海产品。在本研究中，从泡菜中分离出两株新型乳酸杆菌菌株，并研究了它们的抗AD作用。根据共轭亚油酸产生活性，从泡菜中分离出的乳酸杆菌被鉴定为约氏乳杆菌（JBCC105645和JBCC105683）。使用二硝基氟苯（DNFB）在BALB/c小鼠中诱导出AD样皮肤病变。评估小鼠的耳部肿胀、组织学分析和血清免疫球蛋白E（IgE）水平，以研究乳酸杆菌的抗AD作用。还评估了分化簇（CD）4 T细胞的细胞因子产生以及巨噬细胞的白细胞介素（IL）-12产生，以建立乳酸杆菌作用的推定机制。给予JBCC105645或JBCC105683可抑制DNFB处理小鼠的耳部肿胀和血清IgE水平（P<0.05）。值得注意的是，JBCC105645比JBCC105683更有效（P<0.05）。用乳酸杆菌处理还导致DNFB暴露的CD4 T细胞中IL-4产生显著减少，同时干扰素（IFN）-γ产生增加，以及巨噬细胞中IL-12产生增加（P<0.05）。综上所述，从泡菜中分离出的乳酸杆菌可能通过调节CD4 T细胞中IL-4和IFN-γ的产生来抑制小鼠AD样皮肤炎症的发展，推测是通过增强巨噬细胞的IL-12产生来实现的。
