红皮云杉内生真菌抗虫提取物中的次生代谢产物。
Secondary metabolites from anti-insect extracts of endophytic fungi isolated from Picea rubens.
机构信息
Ottawa-Carleton Institute of Chemistry, Carleton University, Ottawa, Ontario, Canada.
出版信息
Phytochemistry. 2010 May;71(7):760-5. doi: 10.1016/j.phytochem.2010.01.015. Epub 2010 Feb 23.
The extracts of a selection of 150 foliar fungal endophytes isolated from Picea rubens (red spruce) needles were screened by LC-MS and assayed for toxicity. Three of these strains that were toxic to the forest pest Choristoneura fumiferana (eastern spruce budworm) in dietary bioassays were selected for further study. Their culture extracts were analyzed by LC-NMR spectroscopy, and the major metabolites were isolated by LC-MS-SPE or PTLC/column chromatography and characterized. The structures were elucidated by spectroscopic analyses including 2D NMR, HRMS and by comparison to literature data. Compounds 1 and 5-7 are hitherto unknown whereas compounds 2 and 3 are natural products described for the first time. Compound 4 is reported for the first time as a fungal metabolite and 8-9 were identified as known fungal metabolites in genera.
从红皮云杉(Picea rubens)针叶中分离出的 150 种叶内真菌内生菌的提取物通过 LC-MS 进行筛选，并进行毒性检测。在膳食生物测定中，这三种对森林害虫舞毒蛾(东部云杉尺蠖)有毒的菌株被选中进行进一步研究。用 LC-NMR 光谱法分析它们的培养物提取物，用 LC-MS-SPE 或 PTLC/柱层析分离主要代谢物，并对其进行鉴定。通过包括 2D NMR、高分辨率质谱和与文献数据的比较在内的光谱分析来阐明结构。化合物 1 和 5-7 是迄今未知的，而化合物 2 和 3 是首次描述的天然产物。化合物 4 是首次报道的真菌代谢产物，化合物 8-9 被鉴定为属中的已知真菌代谢产物。
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