松属内生真菌的抗真菌代谢产物。

Antifungal metabolites from fungal endophytes of Pinus strobus.

机构信息

Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S5B6, Canada.

出版信息

Phytochemistry. 2011 Oct;72(14-15):1833-7. doi: 10.1016/j.phytochem.2011.05.003. Epub 2011 May 31.

DOI:10.1016/j.phytochem.2011.05.003

PMID:21632082

Abstract

The extracts of five foliar fungal endophytes isolated from Pinus strobus (eastern white pine) that showed antifungal activity in disc diffusion assays were selected for further study. From these strains, the aliphatic polyketide compound 1 and three related sesquiterpenes 2-4 were isolated and characterized. Compound 2 is reported for the first time as a natural product and the E/Z conformational isomers 3 and 4 were hitherto unknown. Additionally, the three known macrolides; pyrenophorol (5), dihydropyrenophorin (6), and pyrenophorin (7) were isolated and identified. Their structures were elucidated by spectroscopic analyses including 2D NMR, HRMS and by comparison to literature data where available. The isolated compounds 1, 2, and 5 were antifungal against both the rust Microbotryum violaceum and Saccharomyces cerevisae.

摘要

从松属（东方白松）中分离出的 5 种具有抗真菌活性的叶部真菌内生菌提取物在圆盘扩散试验中表现出抗真菌活性，被选中进行进一步研究。从这些菌株中分离并鉴定出了脂族聚酮化合物 1 和三种相关的倍半萜 2-4。化合物 2 是首次作为天然产物被报道，其 E/Z 构象异构体 3 和 4 在此前是未知的。此外，还分离和鉴定了三种已知的大环内酯化合物：pyrenophorol（5）、dihydropyrenophorin（6）和 pyrenophorin（7）。通过包括 2D NMR、高分辨质谱和与现有文献数据的比较等光谱分析确定了它们的结构。分离得到的化合物 1、2 和 5 对锈菌 Microbotryum violaceum 和酿酒酵母均具有抗真菌活性。