黄孢原毛平革菌锰过氧化物酶对木质素的体外解聚作用
In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium.
作者信息
Wariishi H, Valli K, Gold M H
机构信息
Dept. of Chemical & Biological Sciences, Oregon Graduate Institute of Science & Technology, Beaverton 97006-1999.
出版信息
Biochem Biophys Res Commun. 1991 Apr 15;176(1):269-75. doi: 10.1016/0006-291x(91)90919-x.
Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different 14C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of 14C-sidechain-labeled syringyl lignin, a 14C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of 14C-sidechain- and 14C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzo-quinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.
均相锰过氧化物酶催化了四种不同的14C标记合成木质素制剂的体外部分解聚。凝胶渗透图谱表明，14C侧链标记的紫丁香基木质素、14C侧链标记的紫丁香基-愈创木基共聚物（被子植物木质素）发生了显著解聚，14C侧链和14C环标记的愈创木基木质素（裸子植物木质素）也发生了解聚。3,5-二甲氧基-1,4-苯醌、3,5-二甲氧基-1,4-对苯二酚和紫丁香醛被鉴定为紫丁香基和紫丁香基-愈创木基木质素的降解产物。这些结果表明，锰过氧化物酶在黄孢原毛平革菌对木质素的解聚中起重要作用。
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