State Key Laboratory for Modification of Chemical Fibres and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, P R China.
J Biomater Sci Polym Ed. 2011;22(8):1069-82. doi: 10.1163/092050610X498204. Epub 2010 Jul 2.
Silk fibroin (SF)-hydroxybutyl chitosan (HBC) blend nanofibrous scaffolds were fabricated using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and trifluoroacetic acid (TFA) as solvents to biomimic the native ECM by electrospinning. SEM results showed that the average nanofibrous diameter increased when the content of HBC was raised from 20% to 100%. Whereas water contact angle measurements confirmed that SF/HBC nanofibrous scaffolds with different weight ratios were of good hydrophilicity. Both the tensile strength and the elongation at break were improved obviously when the weight ratio of SF to HBC was 20:80. (13)C-NMR clarified that SF and HBC molecules existed in H-bond interactions, but HBC did not induce SF conformation to transform from random coil form to β-sheet structure. Moreover, the use of genipin vapour not only induced conformation of SF to convert from random coil to β-sheet structure but also acted as a cross-linking agent for SF and HBC. Cell viability studies demonstrated that SF/HBC nanofibrous scaffolds presented good cellular compatibility. Thus, electrospun SF/HBC blended nanofibres may provide an ideal biomimic tissue-engineering scaffold.
丝素蛋白（SF）-羟丁基壳聚糖（HBC）共混纳米纤维支架是采用 1,1,1,3,3,3-六氟-2-丙醇（HFIP）和三氟乙酸（TFA）作为溶剂，通过静电纺丝来仿生天然 ECM。SEM 结果表明，当 HBC 的含量从 20%增加到 100%时，平均纳米纤维直径增加。而水接触角测量则证实，具有不同重量比的 SF/HBC 纳米纤维支架具有良好的亲水性。当 SF 与 HBC 的重量比为 20:80 时，拉伸强度和断裂伸长率明显提高。（13）C-NMR 表明 SF 和 HBC 分子存在氢键相互作用，但 HBC 不会诱导 SF 构象从无规卷曲形式转变为 β-折叠结构。此外，使用京尼平蒸气不仅诱导 SF 构象从无规卷曲转变为 β-折叠结构，而且还充当 SF 和 HBC 的交联剂。细胞活力研究表明，SF/HBC 纳米纤维支架具有良好的细胞相容性。因此，静电纺丝 SF/HBC 共混纳米纤维可能提供一种理想的仿生组织工程支架。
