Hightower Katherine, Marsella Rosanna, Flynn-Lurie Alison
College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-126, USA.
Vet Dermatol. 2010 Feb;21(1):88-95. doi: 10.1111/j.1365-3164.2009.00839.x.
Transepidermal water loss (TEWL) was measured as assessment of barrier function in house dust mite-sensitized atopic (n = 24) and normal (n = 21) beagle dogs before and after allergen challenge. Dogs of the two groups were matched for age and housed in the same environment. Ten sites were evaluated including both areas of high and low predisposition to atopic lesion formation. On day 0 (before allergen exposure) and day 3 (3 days after allergen exposure), TEWL measurements were taken using an open-chamber evaporimeter. On day 0, significant differences (one-way ANOVA) were found between normal and atopic dogs in the chin, pinna, periocular region, axilla, antebrachium and thorax. Within the atopic group, a significant increase (one-way ANOVA) in TEWL was found in the axilla on day 3. Such a difference was particularly marked in young dogs (chin, pinna, axilla, antebrachium and groin). Within the normal group, allergen challenge did not change TEWL except for the chin. When atopic and nonatopic sites were compared within the atopic group, a significant difference in TEWL was found on day 0. A two-way ANOVA showed a significant effect of site, a significant effect of time but no site x time (i.e. allergen exposure) interaction. It is concluded that the house dust mite-sensitized atopic beagle dogs used in this study have altered barrier function in 'atopic' areas and that such differences are more evident in young individuals and are aggravated by allergen exposure.
通过测量经表皮水分流失（TEWL）来评估屋尘螨致敏的特应性比格犬（n = 24）和正常比格犬（n = 21）在变应原激发前后的屏障功能。两组犬在年龄上相匹配，并饲养在相同环境中。评估了10个部位，包括特应性病变形成倾向高和低的区域。在第0天（变应原暴露前）和第3天（变应原暴露后3天），使用开放式蒸发仪进行TEWL测量。在第0天，正常犬和特应性犬在下巴、耳廓、眼周区域、腋窝、前臂和胸部存在显著差异（单因素方差分析）。在特应性组中，第3天腋窝处的TEWL显著增加（单因素方差分析）。这种差异在幼犬（下巴、耳廓、腋窝、前臂和腹股沟）中尤为明显。在正常组中，除下巴外，变应原激发并未改变TEWL。在特应性组内比较特应性和非特应性部位时，第0天TEWL存在显著差异。双向方差分析显示部位有显著影响、时间有显著影响，但不存在部位×时间（即变应原暴露）交互作用。得出的结论是，本研究中使用的屋尘螨致敏的特应性比格犬在“特应性”区域的屏障功能发生了改变，且这种差异在年轻个体中更明显，并因变应原暴露而加重。
