Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
Fertil Steril. 2010 May 15;93(8):2594-601, 2601.e1-9. doi: 10.1016/j.fertnstert.2009.12.053. Epub 2010 Feb 26.
To suggest an alternative strategy for deriving histocompatible stems cells without undertaking genetic manipulation.
Prospective approach using an animal model.
Stem cell and bioevaluation laboratory, Seoul National University.
ANIMAL(S): F1 (C57BL6 X DBA2) and outbred (ICR) mice.
INTERVENTION(S): Ovarian stroma cells of less than 40 mum in diameter were subcultured with fibroblast monolayer, and colony-forming cells were characterized.
MAIN OUTCOME MEASURE(S): Stemness, genotype, and imprinted gene methylation.
RESULT(S): Two-lines of colony-forming cells were established, which expressed markers specific for embryonic stem cells (ESC) and formed embryoid bodies and teratomas. Complete matching of microsatellite markers with the cell donor strain confirmed their establishment from ovarian tissue, and identification of both homozygotic and heterozygotic chromosomes raised the possibility of their derivation from parthenogenetic oocytes. However, the use of cells smaller than mature oocytes for primary culture, the difference in imprinted gene methylation compared with parthenogenetic ESCs, and failure to establish the ESC-like cells by primary follicle culture collectively suggested the irrelevancy to gametes.
CONCLUSION(S): Coculture of adult ovarian cells with somatic fibroblasts can yield colony-forming cells having ESC-like activity, which may provide an alternative for establishing autologous stem cells from adults that can be obtained without genetic manipulation.
提出一种替代策略，从不需要进行基因操作的组织中获得组织相容性干细胞。
使用动物模型的前瞻性方法。
成体干细胞和生物评价实验室，首尔国立大学。
F1（C57BL6 X DBA2）和远交系（ICR）小鼠。
小于 40 微米的卵巢基质细胞与成纤维细胞单层共培养，并对集落形成细胞进行特征分析。
干性、基因型和印迹基因甲基化。
建立了两条集落形成细胞系，其表达胚胎干细胞（ESC）的特异性标记，并形成类胚体和畸胎瘤。微卫星标记与细胞供体株的完全匹配证实了它们来源于卵巢组织，而同源和异源染色体的鉴定增加了它们来源于孤雌生殖卵母细胞的可能性。然而，由于初级培养使用的细胞小于成熟卵母细胞，印迹基因甲基化与孤雌生殖 ESC 相比存在差异，以及初级卵泡培养未能建立 ESC 样细胞，这共同表明与配子无关。
成年卵巢细胞与成纤维细胞共培养可产生具有 ESC 样活性的集落形成细胞，这可能为从成年人中建立自体干细胞提供一种替代方法，而无需进行基因操作即可获得。
