Department of Plant Biology, Institute of Biology, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
Talanta. 2010 Apr 15;81(1-2):100-8. doi: 10.1016/j.talanta.2009.11.043. Epub 2009 Nov 24.
Chemical profiles of a representative set of 49 propolis ethanolic extracts collected worldwide (North and South America, Europe, Asia and Oceania) were obtained via easy ambient sonic-spray ionization mass spectrometry (EASI-MS). This simple and easily implemented fingerprinting technique analyses directly (without any pre-separation or sample manipulation) a tiny droplet of the ethanolic extract placed on a inert surface under ambient conditions. Data acquisition took about a minute per sample with no substantial sample carry-over. Extraction of propolis with ethanol by using an ultrasonic bath method gave EASI-MS data similar to the traditional maceration method, reducing total analysis time for the crude propolis resin from a week to just ca 1h. Principal component analysis of the EASI-MS data is shown to group samples according to the plant sources of their resins, which characterizes their geographical origin.
采用简单的环境声喷雾电离质谱（EASI-MS）获得了来自世界各地（北美、南美、欧洲、亚洲和大洋洲）的 49 种代表性蜂胶乙醇提取物的化学特征图谱。该简单且易于实现的指纹图谱技术直接分析（无需任何预分离或样品处理）置于环境条件下惰性表面上的小乙醇提取物液滴。每个样品的采集时间约为 1 分钟，几乎没有样品残留。使用超声浴法用乙醇提取蜂胶可得到与传统浸渍法相似的 EASI-MS 数据，将粗蜂胶树脂的总分析时间从一周缩短至约 1 小时。EASI-MS 数据的主成分分析显示，根据树脂的植物来源对样品进行分组，从而确定其地理来源。
