Defence Research & Development Establishment, Gwalior 474002, Madhya Pradesh, India.
Talanta. 2010 Apr 15;81(1-2):703-8. doi: 10.1016/j.talanta.2010.01.007. Epub 2010 Jan 11.
Ricin is a scheduled chemical warfare agent and biological warfare agent. Attempts were made for the detection of ricin in water samples by utilizing amperometric immunosensors. These electrodes were made by mixing Paraffin oil with graphite powder and multiwalled carbon nanotubes. The graphite paste electrode (CPE) and multiwalled carbon nanotubes paste electrode (MWCNTPE) were tested for their ability to detect 1-naphthol. A sandwich enzyme linked immunosorbent assay system was used to detect ricin. The detection limit for both electrodes was compared. It was found that the response of amperometric sensor is proportional to the ricin concentration in both the cases and is linear in the range 0.625-25 ng/ml for MWCNTPE and 2.5-25 ng/ml for CPE. The SEM showed that the MWCNTPE has revealed crevices/voids in which the antibodies may get trapped. Spectroscopic experiments proved that MWCNTPE adsorbs antibodies better than CPE. The high sensitivity of MWCNTPE was attributed to its better electrochemical properties rather than to its efficiency to adsorb antibodies.
蓖麻毒素是一种已被列管的化学战剂和生物战剂。曾有研究尝试利用电流免疫传感器来检测水样中的蓖麻毒素。这些电极是通过将石蜡油与石墨粉和多壁碳纳米管混合制成的。对石墨糊电极（CPE）和多壁碳纳米管糊电极（MWCNTPE）进行了检测，以评估它们检测 1-萘酚的能力。采用三明治酶联免疫吸附测定系统检测蓖麻毒素。比较了两种电极的检测限。结果发现，两种情况下电流传感器的响应与蓖麻毒素浓度成正比，MWCNTPE 的线性范围为 0.625-25 ng/ml，CPE 的线性范围为 2.5-25 ng/ml。扫描电子显微镜显示，MWCNTPE 表面存在缝隙/空隙，抗体可能被困在其中。光谱实验证明，MWCNTPE 比 CPE 更能有效地吸附抗体。MWCNTPE 的高灵敏度归因于其更好的电化学性能，而不是其吸附抗体的效率。
