Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, PR China.
Mol Immunol. 2010 Apr;47(7-8):1601-7. doi: 10.1016/j.molimm.2010.01.024. Epub 2010 Mar 1.
The S100A12 gene belongs to the S100 family of genes, which are specific to vertebrates. It is involved in many inflammatory diseases of human and has been considered as a powerful diagnostic gene. In the present study, we identified the porcine S100A12 (pS100A12) gene, provided evidence that pS100A12 is located on chromosome 4 and is closely linked to SW512. We show that pS100A12 is expressed preferentially in immune organs/tissues, e.g., bone marrow, spleen, and inguinal lymph nodes. Expression of the pS100A12 gene is dramatically induced in porcine whole blood cultures by both lipopolysaccharide (LPS) and polyinosine-polycytidylic acid (Poly(I:C)). Elevated expression of pS100A12 is also correlated with in vivo infection with porcine circovirus type 2 (PCV-2) from at least 48h post infection. By analyzing a series of pS100A12 promoter reporter constructs, we have defined two crucial regions (-1013 to -590, -135 to -50) that are responsible for LPS- and Poly(I:C)-induced transcriptional activation, and demonstrated that the LPS/Poly(I:C)-PKC-C/EBPb-pS100A12 pathway may play a critical role in the transcription of the pS100A12.
S100A12 基因属于 S100 基因家族，是脊椎动物所特有的。它参与了许多人类的炎症性疾病，并被认为是一种强大的诊断基因。在本研究中，我们鉴定了猪 S100A12（pS100A12）基因，证明 pS100A12 位于染色体 4 上，与 SW512 紧密连锁。我们表明 pS100A12 优先在免疫器官/组织中表达，例如骨髓、脾脏和腹股沟淋巴结。猪全血培养物中的脂多糖（LPS）和聚肌苷酸-聚胞苷酸（Poly（I：C））均可显著诱导 pS100A12 的表达。pS100A12 的表达升高也与感染猪圆环病毒 2 型（PCV-2）相关，至少在感染后 48 小时。通过分析一系列 pS100A12 启动子报告基因构建体，我们定义了两个负责 LPS 和 Poly（I：C）诱导转录激活的关键区域（-1013 至-590、-135 至-50），并证明 LPS/Poly（I：C）-PKC-C/EBPb-pS100A12 途径可能在 pS100A12 的转录中发挥关键作用。
