Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Brazil.
Anaerobe. 2011 Oct;17(5):257-8. doi: 10.1016/j.anaerobe.2011.06.002. Epub 2011 Jun 23.
A rapid PCR approach was developed to detect Porphyromonas gulae strains from subgingival samples of dogs with and with periodontitis. The presence of P. gulae was observed in 92% and 56%, respectively, in dogs with and without periodontitis. The new primer pair was specific to detect this microorganism, and this technique could be used to evaluate a correlation between periodontitis and P. gulae in companion animals.
研究人员开发了一种快速 PCR 方法,用于检测伴或不伴牙周炎的犬龈下样本中的牙龈卟啉单胞菌菌株。结果显示,伴牙周炎和不伴牙周炎的犬中,牙龈卟啉单胞菌的检出率分别为 92%和 56%。新的引物对特异性检测到这种微生物,该技术可用于评估伴侣动物的牙周炎与牙龈卟啉单胞菌之间的相关性。