Lott Timothy J, Frade João P, Lockhart Shawn R
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Eukaryot Cell. 2010 Apr;9(4):619-25. doi: 10.1128/EC.00002-10. Epub 2010 Feb 26.
The human commensal yeast Candida glabrata is becoming increasingly important as an agent of nosocomial bloodstream infection. However, relatively little is known concerning the genetics and population structure of this species. We have analyzed 230 incident bloodstream isolates from previous and current population-based surveillance studies by using multilocus sequence typing (MLST). Our results show that in the U.S. cities of Atlanta, GA; Baltimore, MD; and San Francisco, CA during three time periods spanning 1992 to 2009, five populations of C. glabrata bloodstream isolates are defined by a relatively small number of sequence types. There is little genetic differentiation in the different C. glabrata populations. We also show that there has been a significant temporal shift in the prevalence of one major subtype in Atlanta. Our results support the concept that both recombination and clonality play a role in the population structure of this species.
人体共生酵母光滑念珠菌作为医院血流感染的病原体正变得越来越重要。然而，关于该物种的遗传学和种群结构，人们所知相对较少。我们通过多位点序列分型（MLST）分析了来自以往和当前基于人群的监测研究中的230株血流感染分离株。我们的结果表明，在1992年至2009年的三个时间段内，在美国佐治亚州亚特兰大市、马里兰州巴尔的摩市和加利福尼亚州旧金山市，光滑念珠菌血流感染分离株的五个种群由相对较少的序列类型定义。不同的光滑念珠菌种群之间几乎没有遗传分化。我们还表明，亚特兰大一种主要亚型的流行率存在显著的时间变化。我们的结果支持这样一种观点，即重组和克隆性在该物种的种群结构中都发挥着作用。
