Department of Family Medicine, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei, Taiwan.
Nephrol Dial Transplant. 2010 Aug;25(8):2730-7. doi: 10.1093/ndt/gfq082. Epub 2010 Feb 26.
T-cell damage by increased oxidative stress in end-stage renal disease (ESRD) patients undergoing chronic haemodialysis (HD) led to the increased T-cell apoptosis and the alteration of surface markers and Th1/Th2 ratio in CD4(+) T lymphocytes. Antioxidant electrolysed-reduced water (ERW) was used as the dialysate in ESRD patients undergoing chronic HD to test for improved oxidative stress-related T-cell apoptosis, alterations of surface markers and intracellular cytokine profile.
We evaluated apoptosis formation by annexin V, CD25-related surface markers, and cytokine ratio of Th1/Th2 in CD4(+) T lymphocytes and Tc1/Tc2 in CD8(+) T lymphocytes of 42 ESRD patients haemodialysed with ERW for 1 year.
In comparison to 12 healthy individuals, the ESRD patients had more T-cell apoptosis and less CD3(+), CD4(+) and CD8(+) T cells and CD25/CD69/CD94/CD3(+) phenotypes at baseline. Lower intracellular IL-2 and IFN-gamma levels in the Th1/CD4(+) and Tc1/CD8(+) cells and higher intracellular IL-4, IL-6 and IL-10 levels in the Th2/CD4(+) and Tc2/CD8(+) cells were also noted in the ESRD patients. After a 1-year ERW treatment, the patients had a decrease in T-cell apoptosis and increases in CD3(+), CD4(+) and CD8(+) cell numbers and CD25/CD69/CD94/CD3(+) phenotypes in the T cells. The intracellular IL-2 and IFN-gamma levels in the Th1/Tc1 cells significantly (P < 0.05) increased and the intracellular IL-4, IL-6 and IL-10 levels in the Th2/Tc2 cells decreased. Furthermore, the Th1/Th2 and Tc1/Tc2 cytokine ratios were improved toward a normal status.
One-year ERW treatment effectively ameliorated T-cell apoptosis, altered CD25-related surface markers and intracellular cytokine profile in the HD patients.
终末期肾病（ESRD）患者在接受慢性血液透析（HD）时，由于氧化应激增加导致 T 细胞损伤，从而导致 CD4+T 淋巴细胞凋亡增加，表面标志物改变以及 Th1/Th2 比例失衡。抗氧化电解还原水（ERW）被用作 ESRD 患者进行慢性 HD 的透析液，以测试改善与氧化应激相关的 T 细胞凋亡，改变表面标志物和细胞内细胞因子谱。
我们评估了 42 名接受 ERW 血液透析治疗 1 年的 ESRD 患者的 CD4+T 淋巴细胞中 T 细胞凋亡的形成，通过 annexin V、CD25 相关表面标志物以及 Th1/Th2 细胞因子比值和 CD8+T 淋巴细胞中的 Tc1/Tc2。
与 12 名健康个体相比，ESRD 患者在基线时具有更多的 T 细胞凋亡以及更少的 CD3+，CD4+和 CD8+T 细胞以及 CD25/CD69/CD94/CD3+表型。ESRD 患者的 Th1/CD4+和 Tc1/CD8+细胞中的细胞内 IL-2 和 IFN-γ水平较低，而 Th2/CD4+和 Tc2/CD8+细胞中的细胞内 IL-4，IL-6 和 IL-10 水平较高。在 ERW 治疗 1 年后，患者的 T 细胞凋亡减少，CD3+，CD4+和 CD8+细胞数量以及 T 细胞中的 CD25/CD69/CD94/CD3+表型增加。Th1/Tc1 细胞中的细胞内 IL-2 和 IFN-γ水平显著增加（P <0.05），而 Th2/Tc2 细胞中的细胞内 IL-4，IL-6 和 IL-10 水平降低。此外，Th1/Th2 和 Tc1/Tc2 细胞因子比值改善至正常状态。
经过一年的 ERW 治疗，有效地改善了 HD 患者的 T 细胞凋亡，改变了 CD25 相关表面标志物和细胞内细胞因子谱。
