Zaizar-Fregoso Sergio A, Paz-Michel Brenda A, Rodriguez-Hernandez Alejandrina, Paz-Garcia Juan, Aurelien-Cabezas Nomely S, Tiburcio-Jimenez Daniel, Melnikov Valery, Murillo-Zamora Efren, Delgado-Enciso Osiris G, Cabrera-Licona Ariana, Guzman-Esquivel José, Barajas-Saucedo Carlos E, Rodriguez-Sanchez Iram P, Martinez-Fierro Margarita L, Moy-López Norma A, Lara-Esqueda Agustin, Guzman-Muñiz Jorge, Delgado-Machuca Marina, Delgado-Enciso Ivan
Facultad de Medicina, Facultad de Psicologia, Universidad de Colima, Colima 28040, Mexico.
Instituto Estatal de Cancerología, Servicios de Salud del Estado de Colima, Colima 28085, Mexico.
Evid Based Complement Alternat Med. 2022 Jun 2;2022:1717614. doi: 10.1155/2022/1717614. eCollection 2022.
Rheumatoid arthritis is globally present in about 1% of the population. This autoinflammatory disease modifies the connective tissue, causing pain and inflammation of the joints. Over time, it causes the loss of joint cartilage and bone mass, decreasing the patient's quality of life. Treatment options now available either give symptomatic alleviation or alter the disease process. Nonetheless, adherence to chronic treatment is typically limited due to adverse effects. As a result, new therapy approaches, such as systemic administration of neutral electrolyzed saline to improve patients' quality of life, are being investigated. The study is a randomized prospective preclinical trial with a single-blind and a 4-arm parallel group using a collagen-induced mice model to generate rheumatoid arthritis. It was carried out on 36 male BALB/c mice, with the primary outcome measure being a scoring system for histopathologic assessment. When all groups are compared, there are significant differences. In addition, the animal model was validated by the healthy group. The animals treated with neutral electrolyzed saline had much less cartilage degradation, bone erosion, pannus development, and inflammation than the placebo-treated mice. Serum IL-6 levels were evaluated in parallel with disease severity expressed as synovitis grading of the affected joints. Spearman's rank correlation coefficient (Rs) = 0.399 (=0.016) between serum IL-6 levels and the synovitis grading suggests a direct correlation between IL-6 production and disease severity. An additional trial of 20 male BALB/c mice (10 treated with placebo and 10 with neutral electrolyzed saline for 30 days) showed no clinical nor histopathological evidence of adverse effects. According to histopathological and blood test results, we conclude that neutral electrolyzed saline minimizes mechanical and inflammatory damage to the joint and may be helpful as an alternative to rheumatoid arthritis therapy.
类风湿性关节炎在全球约1%的人口中存在。这种自身炎症性疾病会改变结缔组织，导致关节疼痛和炎症。随着时间的推移，它会导致关节软骨和骨量的流失，降低患者的生活质量。目前可用的治疗方案要么是缓解症状，要么是改变疾病进程。然而，由于不良反应，慢性治疗的依从性通常有限。因此，正在研究新的治疗方法，如全身给予中性电解盐水以改善患者的生活质量。该研究是一项随机前瞻性临床前试验，采用单盲和四臂平行组设计，使用胶原诱导的小鼠模型来诱发类风湿性关节炎。研究对36只雄性BALB/c小鼠进行，主要结局指标是组织病理学评估的评分系统。当对所有组进行比较时，存在显著差异。此外，健康组验证了该动物模型。与接受安慰剂治疗的小鼠相比，接受中性电解盐水治疗的动物软骨降解、骨侵蚀、血管翳形成和炎症要少得多。血清IL-6水平与以受影响关节滑膜炎分级表示的疾病严重程度同时进行评估。血清IL-6水平与滑膜炎分级之间的Spearman等级相关系数（Rs）=0.399（P=0.016），表明IL-6产生与疾病严重程度之间存在直接相关性。另外一项对20只雄性BALB/c小鼠（10只接受安慰剂治疗，10只接受中性电解盐水治疗30天）的试验未显示出临床或组织病理学上的不良反应证据。根据组织病理学和血液检测结果，我们得出结论，中性电解盐水可将对关节的机械性和炎症性损伤降至最低，可能有助于作为类风湿性关节炎治疗的替代方法。
