Teoh S L, Abd Latiff Azian, Das S
Department of Anatomy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Rom J Morphol Embryol. 2010;51(1):91-5.
Momordica charantia (MC) or bitter gourd is widely known for its antidiabetic properties. The aim of the present study was to observe the protective effect of MC extract on the kidneys of streptozotocin-induced diabetic rats. Eighteen male Sprague-Dawley rats (n=18) weighing 200+/-50 g were taken for the study. The study comprised of three groups i.e. a non-diabetic, diabetic untreated and diabetic treated with MC extract, with each group comprising of six (n=6) rats. Diabetes was induced in the overnight fasted rats by intramuscular injection of streptozotocin (50 mg/kg body weight). The MC extract (50 mg/kg body weight) was administered via oral gavage. Both the kidneys were collected on the tenth day following treatment. Histological study using Verhoeff's van Gieson (VvG) and Periodic Acid-Schiff (PAS) stains were performed. The kidneys of the diabetic rats showed thickening of the basement membrane of the Bowman's capsule, edema and hypercellurarity of the proximal tubules, necrosis and hyaline deposits. These features were found to be reversed when the MC extract was administered to the experimental animals. The MC extract acted as an antioxidant thereby preventing the oxidative damage involved in the diabetic kidney. The administration of MC extract prevents oxidative damage in diabetic nephropathy.
苦瓜（MC），即苦瓜，以其抗糖尿病特性而广为人知。本研究的目的是观察苦瓜提取物对链脲佐菌素诱导的糖尿病大鼠肾脏的保护作用。选取18只体重为200±50克的雄性Sprague-Dawley大鼠（n = 18）用于该研究。该研究包括三组，即非糖尿病组、未治疗的糖尿病组和用苦瓜提取物治疗的糖尿病组，每组有六只（n = 6）大鼠。通过肌肉注射链脲佐菌素（50毫克/千克体重）使过夜禁食的大鼠诱发糖尿病。通过口服灌胃给予苦瓜提取物（50毫克/千克体重）。在治疗后的第十天收集双侧肾脏。使用魏尔啸氏-范吉森（VvG）和过碘酸-希夫（PAS）染色进行组织学研究。糖尿病大鼠的肾脏显示出鲍曼囊基底膜增厚、近端小管水肿和细胞增多、坏死以及透明样沉积物。当给实验动物施用苦瓜提取物时，发现这些特征有所逆转。苦瓜提取物起到抗氧化剂的作用，从而防止糖尿病肾脏中涉及的氧化损伤。施用苦瓜提取物可预防糖尿病肾病中的氧化损伤。
