用于未处理人血清中低丰度蛋白质生物标志物定量的纳米磁竞争分析。

Nanomagnetic competition assay for low-abundance protein biomarker quantification in unprocessed human sera.

机构信息

Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA.

出版信息

J Am Chem Soc. 2010 Mar 31;132(12):4388-92. doi: 10.1021/ja910406a.

PMID:20192199

Abstract

A novel giant magnetoresistive sensor and uniform high-magnetic-moment FeCo nanoparticles (12.8 nm)-based detecting platform with minimized detecting distance was developed for rapid biomolecule quantification from body fluids. Such a system demonstrates specific, accurate, and quick detection and quantification of interleukin-6, a low-abundance protein and a potential cancer biomarker, directly in 4 muL of unprocessed human sera. This platform is expected to facilitate the identification and validation of disease biomarkers. It may eventually lead to a low-cost personal medical device for chronic disease early detection, diagnosis, and prognosis.

摘要

开发了一种新型巨磁电阻传感器和基于均匀高磁矩 FeCo 纳米粒子（12.8nm）的检测平台，可实现最小检测距离，用于快速从体液中定量生物分子。该系统能够特异性、准确且快速地检测和定量低丰度蛋白质和潜在癌症生物标志物白细胞介素 6，直接检测未经处理的 4μL 人血清中的白细胞介素 6。该平台有望促进疾病生物标志物的识别和验证。它最终可能会导致用于慢性病早期检测、诊断和预后的低成本个人医疗设备。