Daniel Karen D, Kim Grace Y, Vassiliou Christophoros C, Jalali-Yazdi Farzad, Langer Robert, Cima Michael J
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Lab Chip. 2007 Oct;7(10):1288-93. doi: 10.1039/b705143c. Epub 2007 Jul 20.
By combining the sensing capabilities of nanoscale magnetic relaxation switches (MRS) within multi-reservoir structures, a potentially powerful implantable multiplexed sensor has been developed. MRS are magnetic nanoparticles that decrease the transverse relaxation time (T(2)) of water in the presence of an analyte. The switches encased in polydimethylsiloxane (PDMS) devices with polycarbonate membranes (10 nm pores) have demonstrated in vitro sensing of the beta subunit of human chorionic gonadotrophin (hCG-beta), which is elevated in testicular and ovarian cancer. Devices showed transverse relaxation time (T(2)) shortening by magnetic resonance imaging (MRI) when incubated in analyte solutions of 0.5 to 5 microg hCG-beta mL(-1). The decrease in T(2) was between 9% and 27% (compared to control devices) after approximately 28 h. This prototype device is an important first step in developing an implantable sensor for detecting soluble cancer biomarkers in vivo.
通过将纳米级磁弛豫开关（MRS）的传感能力与多储库结构相结合，已开发出一种具有潜在强大功能的可植入式多路复用传感器。MRS是磁性纳米颗粒，在存在分析物的情况下会降低水的横向弛豫时间（T(2)）。封装在带有聚碳酸酯膜（10纳米孔径）的聚二甲基硅氧烷（PDMS）装置中的开关已在体外对人绒毛膜促性腺激素β亚基（hCG-β）进行了传感测试，hCG-β在睾丸癌和卵巢癌中水平升高。当在0.5至5微克hCG-β mL(-1)的分析物溶液中孵育时，通过磁共振成像（MRI）显示装置的横向弛豫时间（T(2)）缩短。大约28小时后，T(2)的降低幅度在9%至27%之间（与对照装置相比）。这种原型装置是开发用于在体内检测可溶性癌症生物标志物的可植入传感器的重要第一步。
