2005年7月至2008年6月兰州婴幼儿人腺病毒腹泻的分子流行病学研究

[Molecular epidemiology of human adenovirus diarrhea among infants and young children in Lanzhou from July 2005 to June 2008].

作者信息

Qi Hong-mei, Jin Yu, Duan Zhao-jun, Ye Xin-hua, Cheng Wei-xia, Zhu Lin

机构信息

Department of Pediatrics, First Hospital, Lanzhou University, Lanzhou 730000, China.

出版信息

Zhonghua Er Ke Za Zhi. 2009 Dec;47(12):922-5.

PMID:20193145

Abstract

OBJECTIVE

Gastroenteritis is a major cause of childhood morbidity and mortality worldwide. Adenovirus AdV is recognized to be one of the most important pathogens associated with severe dehydrating gastroenteritis. Studies reported elsewhere have shown that about 8%-10% of cases with infantile diarrhea are caused by AdV and in some areas AdV diarrhea even occurred in the form of outbreaks. Studies have confirmed that AdV infections are also very common in infants and young children in China. This study aimed to investigate the molecular epidemiologic characteristics of human adenovirus diarrhea among infants and children with acute diarrhea in Lanzhou, Gansu province, China.

METHOD

Stool specimen and case information were collected from both outpatients and inpatients with acute diarrhea in Lanzhou. Polymerase chain reaction was used to detect AdV in stool specimens. The subjects included 709 urban children and 180 rural children, their age ranged from 19 d to 60 months.

RESULT

Of the 889 cases, 43 (4.8%) were found positive for AdV. AdV was detected in 14 of 257 (5.4%) cases seen from July 2005 to June 2006, in 4 of 286 cases (1.4%) seen from July 2006 to June 2007. During the period of July 2007 to June 2008, adenovirus was detected in 346 specimens, the positive rate was 7.2% (25/346). AdV detection rates of the three-year period were significantly different. The major AdV subtypes detected were adenovirus (subgenus F) Ad40, Ad41 with a positive rate of 3.8% (34/889), followed by non-enteric adenovirus (Ad12, Ad18, Ad31, Ad2, Ad5, Ad6, Ad7) with a positive rate of 1.0% (9/889) in Lanzhou during the 3 years. Most of the AdV-positive specimens showed Ad41 group F (67.4%, 29/43) as the major epidemic strains, and Ade infection mainly occurred in children under one year of age and no seasonal cluster was found.

CONCLUSION

Adenovirus was one of the major etiological agent of viral diarrhea among infants and children in Lanzhou between 2005 and 2008. Ad41 was the prodomiment serotype.

摘要

目的

在全球范围内，胃肠炎是儿童发病和死亡的主要原因。腺病毒（AdV）被认为是与严重脱水型胃肠炎相关的最重要病原体之一。其他地方的研究报告显示，约8%-10%的婴儿腹泻病例由AdV引起，在某些地区，AdV腹泻甚至以暴发形式出现。研究证实，AdV感染在中国婴幼儿中也非常普遍。本研究旨在调查中国甘肃省兰州市急性腹泻婴幼儿人群中人类腺病毒腹泻的分子流行病学特征。

方法

收集兰州市急性腹泻门诊和住院患者的粪便标本及病例信息。采用聚合酶链反应检测粪便标本中的AdV。研究对象包括709名城市儿童和180名农村儿童，年龄范围为19天至60个月。

结果

889例病例中，43例（4.8%）AdV检测呈阳性。2005年7月至2006年6月的257例病例中有14例（5.4%）检测到AdV，2006年7月至2007年6月的286例病例中有4例（1.4%）检测到AdV。在2007年7月至2008年6月期间，346份标本中检测到腺病毒，阳性率为7.2%（25/346）。三年期间的AdV检测率有显著差异。检测到的主要AdV亚型为腺病毒（F亚属）Ad40、Ad41，阳性率为3.8%（34/889），其次是非肠道腺病毒（Ad12、Ad18、Ad31、Ad2、Ad5、Ad6、Ad7），三年间在兰州的阳性率为1.0%（9/889）。大多数AdV阳性标本显示Ad41 F组（67.4%，29/43）为主要流行株，Ade感染主要发生在1岁以下儿童，未发现季节性聚集现象。