Wang Yong-Xia, Li Dan-Di, Jin Yu, Zhang Qing, Wang Hong, Kong Xiang-Yu, Li Yu-Ning, Duan Zhao-Jun
First Clinical Medical College of Lanzhou University, Gangsu 730000, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2012 Feb;26(1):8-10.
To investigate the clinical and molecular epidemiology characteristics of calicivirus and adenovirus in children for viral diarrhea in Lanzhou.
Stool samples were collected from 295 children with diarrhea at the First Hospital of Lanzhou University, Gansu Province,China, between July 2010 and June 2011. Reverse transcription-polymerase chain reaction (RT-PCR) or PCR were used to detected calicivirus and adenovirus. The adenovirus positive samples were typed by nested PCR and multiple PCR.
Of the 295 specimens, 13.2% (39/295) were positive for calicivirus, and 5.1% (15/295) were adenovirus. Typing and Phylogenetic analysis revealed that novirus GII-3 and adenovirus 41 were the dominant strains. Both calicivirus and adenovirus predominately affect children under the age of 2. In seasonal distribution, there was no obvious peak.
Human calicivirus and adenovirus are important pathogens of viral diarrhea,it is important to develop long-term systematic surveillance.
