Liu Li-ying, Qian Yuan, Zhang You, Jia Li-ping, Dong Hui-jin, Deng Jie
Laboratory of Virology, Capital Institute of Pediatrics, Beijing, China.
Zhonghua Er Ke Za Zhi. 2012 Jun;50(6):450-4.
The study was designed to evaluate adenovirus infection in hospitalized children with diarrhea.
Stool specimens were collected from 519 hospitalized children with diarrhea during 2010, including those defined as community-acquired diarrhea (CAD) who developed diarrhea symptoms within 48 hours after admission, and those defined as hospital-acquired diarrhea (HAD) whose symptoms of diarrhea occurred beyond 48 hours after admission. PCR was employed to identify adenovirus in fecal samples by using universal primers for adenoviruses of all types, and specific primers for adenovirus group F. PCR products with expected size were sequenced for adenovirus typing. Clinical data for children with adenovirus positive specimens were analyzed.
A total of 519 hospitalized children, including 289 with CAD and 230 with HAD, were enrolled in the study. Out of 519 stool specimens, 76 showed PCR products with expected 301 bp and identified as adenovirus by sequencing, and the overall positive rate was 14.6%. Out of 289 CAD samples, 43 were positive (positive rate was 14.9%). Of them, 20 were identified as enteric adenovirus infection (adenovirus type 41, Ad41). Thirty-three out of 230 HAD samples were positive (positive rate was 14.3%). Of them, 13 were characterized as enteric adenovirus infection (one was Ad40 and others were Ad41). Ad41 in this study could be divided into two genotypes by phylogenetic tree analysis. Non-enteric adenoviruses were identified in 43 specimens (43/76, 56.6%) including 5 of serotype 1, 8 of serotype 2, 15 of serotype 3, 10 of serotype 7, 1 of serotype 12, and 4 of serotype 31. In this study, the positive rate of adenovirus between CAD children and HAD children did not differ (χ(2) = 0.03, P > 0.05), while the positive rate of enteric adenovirus was high in CAD children.
Adenovirus infection was the main cause of diarrhea in hospitalized children. In this study, the positive rate of adenovirus was similar between children with CAD and with HAD. Enteric adenovirus (adenovirus group F) was the most common adenovirus serotype detected in 2010 in Beijing, and Ad41 was the dominant type.
本研究旨在评估住院腹泻儿童的腺病毒感染情况。
收集了2010年519例住院腹泻儿童的粪便标本，包括那些被定义为社区获得性腹泻（CAD）的儿童，即入院后48小时内出现腹泻症状者，以及那些被定义为医院获得性腹泻（HAD）的儿童，即腹泻症状在入院48小时后出现者。采用聚合酶链反应（PCR），使用针对所有类型腺病毒的通用引物以及腺病毒F组的特异性引物，对粪便样本中的腺病毒进行鉴定。对具有预期大小的PCR产物进行测序以进行腺病毒分型。分析腺病毒阳性标本儿童的临床数据。
本研究共纳入519例住院儿童，其中289例为CAD，230例为HAD。在519份粪便标本中，76份显示出预期的301 bp的PCR产物，经测序鉴定为腺病毒，总体阳性率为14.6%。在289份CAD样本中，43份为阳性（阳性率为14.9%）。其中，20份被鉴定为肠道腺病毒感染（腺病毒41型，Ad41）。230份HAD样本中有33份为阳性（阳性率为14.3%）。其中，13份被鉴定为肠道腺病毒感染（1份为Ad40，其他为Ad41）。通过系统发育树分析，本研究中的Ad41可分为两种基因型。在43份标本（43/76，56.6%）中鉴定出非肠道腺病毒，包括1型5份、2型8份、3型15份、7型10份、12型1份和31型4份。本研究中，CAD儿童和HAD儿童的腺病毒阳性率无差异（χ(2)=0.03，P>0.05），而CAD儿童的肠道腺病毒阳性率较高。
腺病毒感染是住院儿童腹泻的主要原因。本研究中，CAD儿童和HAD儿童的腺病毒阳性率相似。肠道腺病毒（腺病毒F组）是2010年在北京检测到的最常见的腺病毒血清型，且Ad41是优势型。
