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利用杆状病毒表达系统在昆虫细胞中合成破伤风毒素片段C。

Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system.

作者信息

Charles I G, Rodgers B C, Makoff A J, Chatfield S N, Slater D E, Fairweather N F

机构信息

Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent, England.

出版信息

Infect Immun. 1991 May;59(5):1627-32. doi: 10.1128/iai.59.5.1627-1632.1991.

DOI:10.1128/iai.59.5.1627-1632.1991
PMID:2019434
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC257894/
Abstract

The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was expressed intracellularly at a high level and was soluble, allowing it to be purified by affinity chromatography with monoclonal antibody TT08. Purified fragment C from baculovirus was used to immunize mice and was shown to successfully prevent the symptoms of tetanus following a toxin challenge. The ganglioside-binding properties of baculovirus-derived fragment C were compared with those of intact tetanus toxin and native fragment C and were found to be dissimilar.

摘要

杆状病毒表达载体p36C用于在强多角体蛋白启动子的控制下，在昆虫草地贪夜蛾细胞中表达破伤风毒素的C片段。C片段在细胞内高水平表达且可溶，使其能够通过用单克隆抗体TT08进行亲和层析来纯化。从杆状病毒中纯化的C片段用于免疫小鼠，并显示在毒素攻击后成功预防了破伤风症状。将杆状病毒衍生的C片段的神经节苷脂结合特性与完整破伤风毒素和天然C片段的特性进行了比较，发现它们不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2311/257894/d0fc1c1978b2/iai00041-0054-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2311/257894/ebff7406bf70/iai00041-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2311/257894/d0fc1c1978b2/iai00041-0054-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2311/257894/ebff7406bf70/iai00041-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2311/257894/d0fc1c1978b2/iai00041-0054-b.jpg

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