Department of Biochemistry, Basic Medical Science Block, Panjab University, Chandigarh 160014, India.
Mol Cell Biochem. 2010 Jul;340(1-2):231-8. doi: 10.1007/s11010-010-0422-9. Epub 2010 Feb 27.
A very low level of intra-erythrocytic calcium content is very important for normal physiology of cells. In the present study, our main objective was to investigate the effects of atrazine toxicity on Ca(2+) homeostasis and modulation by vitamin E. Experimental animals were orally administered atrazine (300 mg kg(-1) body weight, daily) and vitamin E (100 mg kg(-1) body weight, daily). All studies were carried out for 7, 14, and 21 days in male Wistar rats. Erythrocyte membranes were prepared and were analyzed for lipid peroxidation (LPO) and membrane bound ATPases. Furthermore, Ca(2+) homeostasis was evaluated in erythrocytes. The present findings indicated that atrazine exposure induced oxidative stress which was associated with significant increase in lipid peroxidation (P < 0.05). Vitamin E treatment on the other hand significantly lowered the atrazine-induced lipid peroxidation. The increased LPO following atrazine exposure was accompanied by significant decrease in ATPases (P < 0.05) and disturbed Ca(2+) homeostasis. Furthermore, vitamin E treatment had a beneficial effect by partially restoring ATPases and Ca(2+) homeostasis. The current findings suggest that atrazine exerts its toxic effect by increasing LPO, altering the activity of membrane bound enzymes and disturbing Ca(2+) homeostasis. Vitamin E treatment ameliorated the toxic effects of atrazine suggesting its role as a potential antioxidant.
细胞内钙离子含量极低对于细胞的正常生理功能非常重要。在本研究中，我们的主要目的是研究莠去津毒性对 Ca(2+) 稳态的影响及其与维生素 E 的调节作用。实验动物经口给予莠去津（300mg/kg 体重，每日）和维生素 E（100mg/kg 体重，每日）。所有研究均在雄性 Wistar 大鼠中进行了 7、14 和 21 天。制备红细胞膜并分析脂质过氧化（LPO）和膜结合 ATP 酶。此外，还评估了红细胞中的 Ca(2+) 稳态。本研究结果表明，莠去津暴露诱导氧化应激，与脂质过氧化显著增加（P<0.05）相关。另一方面，维生素 E 处理显著降低了莠去津诱导的脂质过氧化。莠去津暴露后 LPO 的增加伴随着 ATP 酶显著减少（P<0.05）和 Ca(2+) 稳态紊乱。此外，维生素 E 处理具有有益作用，部分恢复了 ATP 酶和 Ca(2+) 稳态。目前的研究结果表明，莠去津通过增加 LPO、改变膜结合酶的活性和扰乱 Ca(2+) 稳态来发挥其毒性作用。维生素 E 处理减轻了莠去津的毒性作用，表明其作为一种潜在的抗氧化剂的作用。
