Lin Jia, Li Hui-Xin, Xia Jun, Li Xue-Nan, Jiang Xiu-Qing, Zhu Shi-Yong, Ge Jing, Li Jin-Long
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Sci Rep. 2016 Apr 26;6:24855. doi: 10.1038/srep24855.
People who drink water contaminated with atrazine (ATR) over many years can experience problems with their cardiovascular system. Lycopene (LYC) has been shown to exhibit cardiovascular disease preventive effects. However, chemopreventive potential of LYC against ATR-induced cardiotoxicity remains unclear. To determine the effects of ATR and/or LYC on heart, mice were treated with ATR (50 mg/kg or 200 mg/kg) and/or LYC (5 mg/kg) by intragastric administration for 21 days. Histopathological and biochemical analyses, including analysis of ion concentrations (Na(+), K(+), Ca(2+) and Mg(2+)), ATPases (Na(+)-K(+)-ATPase, Ca(2+)-ATPase, Mg(2+)-ATPase and Ca(2+)-Mg(2+)-ATPase) activities and the transcription of their subunits, were performed on heart. The results revealed that ATR led to decreased Creative Kinase (CK) activity and increased histological alterations. Furthermore, a significant change in Na(+), K(+) and Ca(2+) content and the down-regulation of Na(+)-K(+)-ATPase and Ca(2+)-ATPase activities and the mRNA expression of their subunits were observed in ATR-exposed mice. Notably, supplementary LYC significantly protected the heart against ATR-induced damage. In conclusion, ATR induced cardiotoxicity by modulating cardiac ATPase activity and the transcription of its subunits, thereby triggering ionic disturbances. However, supplementary LYC significantly combated ATR-induced cardiotoxicity via the regulation of ATPase activity and subunit transcription. Thus, LYC exhibited a significant chemopreventive potential against ATR-induced cardiotoxicity.
长期饮用受莠去津（ATR）污染的水的人，其心血管系统可能会出现问题。番茄红素（LYC）已被证明具有预防心血管疾病的作用。然而，LYC对ATR诱导的心脏毒性的化学预防潜力仍不清楚。为了确定ATR和/或LYC对心脏的影响，通过灌胃给予小鼠ATR（50mg/kg或200mg/kg）和/或LYC（5mg/kg），持续21天。对心脏进行了组织病理学和生化分析，包括离子浓度（Na⁺、K⁺、Ca²⁺和Mg²⁺）分析、ATP酶（Na⁺-K⁺-ATP酶、Ca²⁺-ATP酶、Mg²⁺-ATP酶和Ca²⁺-Mg²⁺-ATP酶）活性及其亚基转录分析。结果显示，ATR导致肌酸激酶（CK）活性降低和组织学改变增加。此外，在暴露于ATR的小鼠中，观察到Na⁺、K⁺和Ca²⁺含量有显著变化，Na⁺-K⁺-ATP酶和Ca²⁺-ATP酶活性及其亚基的mRNA表达下调。值得注意的是，补充LYC可显著保护心脏免受ATR诱导的损伤。总之，ATR通过调节心脏ATP酶活性及其亚基转录诱导心脏毒性，从而引发离子紊乱。然而，补充LYC通过调节ATP酶活性和亚基转录显著对抗ATR诱导的心脏毒性。因此，LYC对ATR诱导的心脏毒性具有显著的化学预防潜力。
