Laboratory Medicine, Qingdao Municipal Hospital, Qingdao, China.
Clin Exp Metastasis. 2010 Mar;27(3):123-32. doi: 10.1007/s10585-010-9309-0. Epub 2010 Mar 2.
The aim of this study was to assess the intratumoral and peritumoral distribution of lymphatic vessel density (LVD) and lymphatic vessel invasion (LVI) in colorectal cancer and their relationships with patients' clinicopathological characteristics and survival. Paraffin sections of 81 primary colorectal cancers were examined by immunohistochemical staining using monoclonal antibody D2-40. Peritumoral LVD was significantly higher than intratumoral LVD (P = 0.000). Both intratumoral LVD and peritumoral LVD were correlated with the presence of LVI (P = 0.006 and P = 0.003, respectively). LVI, intratumoral LVI and peritumoral LVI were identified, respectively in 38, 28 and 32% of the samples investigated. Both intratumoral LVI and peritumoral LVI were correlated with lymph node metastasis (P = 0.030 and P = 0.014, respectively). Lymph node metastasis, the presence of intratumoral LVI and peritumoral LVI were adversely associated with the 5-year overall survival in a univariate analysis (P = 0.001, P = 0.011 and P = 0.017, respectively). Multivariate analysis using Cox proportional hazard model showed that neither intratumoral LVI nor peritumoral LVI was an independent prognostic factor of overall survival. The results of this study demonstrated that intratumoral as well as peritumoral LVI was associated with lymph node metastasis and adverse outcome in colorectal cancer.
本研究旨在评估结直肠癌肿瘤内和肿瘤周围淋巴管密度（LVD）和淋巴管侵犯（LVI）的分布及其与患者临床病理特征和生存的关系。采用单克隆抗体 D2-40 进行免疫组织化学染色，对 81 例原发性结直肠癌的石蜡切片进行检查。肿瘤周围 LVD 明显高于肿瘤内 LVD（P = 0.000）。肿瘤内 LVD 和肿瘤周围 LVD 均与 LVI 的存在相关（P = 0.006 和 P = 0.003）。在所研究的样本中，分别有 38%、28%和 32%的样本存在 LVI、肿瘤内 LVI 和肿瘤周围 LVI。肿瘤内 LVI 和肿瘤周围 LVI 均与淋巴结转移相关（P = 0.030 和 P = 0.014）。单因素分析显示，淋巴结转移、肿瘤内 LVI 和肿瘤周围 LVI 的存在与 5 年总生存率呈负相关（P = 0.001、P = 0.011 和 P = 0.017）。Cox 比例风险模型多因素分析显示，肿瘤内 LVI 和肿瘤周围 LVI 均不是总生存率的独立预后因素。本研究结果表明，肿瘤内和肿瘤周围 LVI 与结直肠癌的淋巴结转移和不良预后相关。
