Biological and Chemical Engineering School, Ningbo Institute of Technology, Zhejiang University, Ningbo, China.
DNA Cell Biol. 2011 Apr;30(4):255-64. doi: 10.1089/dna.2010.1095. Epub 2010 Dec 13.
In vitro studies on the interactions between native herring sperm DNA (HS-DNA) and melamine as well as its related compounds (MARCs), that is, ammeline, ammelide, and cyanuric acid, have been investigated by spectrophotometric, spectrofluorometric, melting temperature, and viscosimetric techniques. It was found that any of the MARCs might interact with HS-DNA by a groove mode of binding via hydrogen bonds. The interaction constants between any of the MARCs and HS-DNA were at 10⁴-10⁵ L mol⁻¹, determined by both spectrophotometric and spectrofluorometric methods. The thermodynamic studies suggested that the interaction processes were exothermic favored (ΔH < 0) and entropy disfavored (ΔS < 0), which indicated that hydrogen bonds might be the main acting force in the binding of MARCs and DNA.
体外研究了天然鲱鱼精子 DNA（HS-DNA）与三聚氰胺及其相关化合物（MARC），即三聚氰胺、三聚氰胺酰胺和三聚氰酸之间的相互作用，采用分光光度法、荧光光谱法、熔点和粘度技术进行了研究。结果发现，MARC 中的任何一种都可能通过氢键通过沟型结合与 HS-DNA 相互作用。通过分光光度法和荧光光谱法测定，任何 MARC 与 HS-DNA 之间的相互作用常数均在 10⁴-10⁵ L mol⁻¹之间。热力学研究表明，相互作用过程是放热有利（ΔH < 0）和熵不利（ΔS < 0），这表明氢键可能是 MARC 和 DNA 结合的主要作用力。
