基于流式细胞术的检测方法，用于测量恶性疟原虫对红细胞的入侵。
A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum.
机构信息
Harvard School of Public Health, Boston, Massachusetts, USA.
出版信息
Am J Hematol. 2010 Apr;85(4):234-7. doi: 10.1002/ajh.21642.
Abstract
Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion.
摘要
疟原虫恶性疟原虫入侵人类红细胞的能力存在差异，这被认为是疾病严重程度的一个重要决定因素。寄生虫的繁殖率和红细胞的选择性都是决定这种可变入侵的重要参数。我们建立了一种基于流式细胞术的方法，可同时计算疟原虫血症和多重感染红细胞的数量。用 DNA 特异性染料 SYBR Green I 染色可定量检测寄生虫发育的环阶段的寄生虫入侵。我们讨论了该方法在体外和体内研究寄生虫入侵方面的应用和局限性。
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