Jang Jin Woo, Kim Ju Yeon, Yoon Jung, Yoon Soo Young, Cho Chi Hyun, Han Eun Taek, An Seong Soo A, Lim Chae Seung
Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul 136-703, Republic of Korea.
Department of Parasitology, Kangwon National University College of Medicine, Chuncheon, Kangwon-do 200-701, Republic of Korea.
ScientificWorldJournal. 2014;2014:536723. doi: 10.1155/2014/536723. Epub 2014 Dec 8.
A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2-37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2-66.0%). High correlation of measured parasitaemia (r=0.98, P<0.05) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients.
开发了一种使用SYBR Green I和CD235A的流式细胞术（FACS）检测恶性疟原虫培养物（恶性疟原虫）的方法，并与吉姆萨染色显微镜检查进行比较。将培养的恶性疟原虫接种到红细胞（RBC）中，以产生0.01%至22.0%的寄生虫血症。FACS分析显示，恶性疟原虫感染的红细胞和未感染的红细胞之间有明显区分。FACS测量的寄生虫血症百分比显示出更高的精度（CV为2.2-37.2%），寄生虫血症灵敏度为0.01%，高于吉姆萨染色显微镜检查（CV为7.2-66.0%）。FACS和吉姆萨染色显微镜分析之间观察到测量的寄生虫血症具有高度相关性（r=0.98，P<0.05）。与吉姆萨染色显微镜分析相比，FACS在所有范围内都观察到了更高水平的寄生虫血症检测。目前报道的使用SYBR Green I和CD235A的FACS方法可能有助于测量治疗患者的寄生虫血症。
