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野外抗疟药物筛选的培养方法。

Culture Method for Antimalarial Drugs Screen in the Field.

机构信息

Faculté de Pharmacie, Malaria Research and Training Center (MRTC), Université des Sciences des Techniques et des Technologies de Bamako, Point G, Postal Box 1805, Bamako, Mali.

Medicines for Malaria Venture (MMV), ICC Building Entrance G Third Floor Route de Pré-Bois 20 Postal Box 1826 CH-1215 Geneva 15 Switzerland.

出版信息

ACS Infect Dis. 2021 Nov 12;7(11):3025-3033. doi: 10.1021/acsinfecdis.1c00262. Epub 2021 Oct 28.

Abstract

and cultivation of has facilitated active research into the malaria parasite toward the quest for basic knowledge and the discovery of effective drug treatments. Such a drug discovery program is currently difficult for simply because of the absence of and cultivation system for its asexual blood stages supporting antimalarial evaluation. Despite availability of artemisinin combination therapies effective on , is being increasingly detected in malaria endemic countries. is responsible for chronic infections and is associated with a high burden of anemia and morbidity. Here, we optimized and adapted conditions under which can be cultured and used for screening antimalarial drugs. Subsequently, this enabled us to test compounds such as artemether, chloroquine, lumefantrine, and quinine for antimalarial activity against .

摘要

和培养促进了对疟原虫的积极研究,以寻求基础知识和发现有效的药物治疗方法。由于缺乏和无性血期培养系统来支持抗疟评估,目前对来说,这样的药物发现计划非常困难。尽管有有效的青蒿素联合疗法,但在疟疾流行的国家中,疟原虫的检出率越来越高。疟原虫负责慢性感染,与贫血和发病率的高负担有关。在这里,我们优化和适应了的培养条件,使其可用于筛选抗疟药物。随后,这使我们能够测试青蒿素、氯喹、咯萘啶和奎宁等化合物对的抗疟活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a44/9974065/a1987ddd94c7/id1c00262_0001.jpg

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