Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 39, Assam, India.
Colloids Surf B Biointerfaces. 2010 Jun 1;77(2):240-5. doi: 10.1016/j.colsurfb.2010.01.033. Epub 2010 Feb 6.
Nanoscale materials are presently gaining much importance for biological applications especially in the field of medicine. The large numbers of nanomaterial based products that are currently being developed - with projected applications in medicine - have inspired a growing interest in exploring their impact on cellular gene expression. The present study examines the effects of silver nanoparticles (NPs) on genes expression in an endeavor to assess the fundamental mechanisms that contribute to silver NP induced programmed cell death. Here, we have used RT-PCR to study the gene expression, flow cytometry analyses to probe the extent of apoptosis (FACS) and atomic force microscopy (AFM) to follow the cell membrane topology change induced by Ag NPs. The gene expression study revealed that Ag NP induced p53-mediated apoptotic pathway through which most of the chemotherapeutic drugs trigger apoptosis (programmed cell death). The results also suggest that Ag NPs could be attributed as therapeutic agent for biomedical and pharmaceutical applications.
目前，纳米材料在生物应用方面越来越受到重视，特别是在医学领域。目前正在开发大量基于纳米材料的产品，预计将在医学领域得到应用，这激发了人们对探索它们对细胞基因表达影响的浓厚兴趣。本研究探讨了银纳米颗粒（NPs）对基因表达的影响，旨在评估导致银 NP 诱导程序性细胞死亡的基本机制。在这里，我们使用 RT-PCR 研究基因表达，流式细胞术分析（FACS）探测细胞凋亡的程度，原子力显微镜（AFM）跟踪 Ag NPs 诱导的细胞膜拓扑结构变化。基因表达研究表明，Ag NP 通过 p53 介导的凋亡途径诱导细胞凋亡（程序性细胞死亡），大多数化疗药物通过该途径触发细胞凋亡。研究结果还表明，Ag NPs 可作为生物医学和制药应用的治疗剂。
