Kim Keuk-Jun, Sung Woo Sang, Suh Bo Kyoung, Moon Seok-Ki, Choi Jong-Soo, Kim Jong Guk, Lee Dong Gun
Department of Microbiology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu, 702-701, South Korea.
Biometals. 2009 Apr;22(2):235-42. doi: 10.1007/s10534-008-9159-2. Epub 2008 Sep 4.
In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (TEM) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH), as a plasma membrane probe, were performed with Candida albicans. The results suggest nano-Ag may exert an antifungal activity by disrupting the structure of the cell membrane and inhibiting the normal budding process due to the destruction of the membrane integrity. The present study indicates nano-Ag has considerable antifungal activity, deserving further investigation for clinical applications.
在本研究中，对银纳米颗粒（纳米银）的抗真菌作用及其作用方式进行了研究。纳米银对所测试的真菌显示出抗真菌作用，且对人红细胞的溶血作用较低。为阐明纳米银的抗真菌作用方式，使用白色念珠菌进行了流式细胞术分析、葡萄糖释放试验、透射电子显微镜（TEM）以及使用作为质膜探针的1,6-二苯基-1,3,5-己三烯（DPH）的膜动力学变化研究。结果表明，纳米银可能通过破坏细胞膜结构并抑制由于膜完整性破坏而导致的正常出芽过程来发挥抗真菌活性。本研究表明纳米银具有相当大的抗真菌活性，值得进一步开展临床应用研究。
