CBS Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;
Persoonia. 2009 Jun;22:56-62. doi: 10.3767/003158509X427808. Epub 2009 Mar 3.
The fungal pathogen Phoma clematidina is used as a biological agent to control the invasive plant species Clematis vitalba in New Zealand. Research conducted on P. clematidina as a potential biocontrol agent against C. vitalba, led to the discovery of two perithecial-forming strains. To assess the diversity of P. clematidina and to clarify the teleomorph-anamorph relationship, phylogenetic analyses of 18 P. clematidina strains, reference strains representing the Phoma sections in the Didymellaceae and strains of related species associated with Clematis were conducted. Partial sequences of the ITS1, ITS2 and 5.8S rRNA gene, the ss-tubulin gene and 28S rRNA gene were used to clarify intra- and inter-species relationships. These analyses revealed that P. clematidina resolves into three well-supported clades which appear to be linked to differences in host specificity. Based on these findings, Didymella clematidis is newly described and the descriptions of P. clematidina and D. vitalbina are amended.
真菌病原菌垂序商陆Phoma clematidina 被用作生物制剂来控制新西兰的入侵植物物种Clematis vitalba。对 P. clematidina 作为防治 C. vitalba 的潜在生防剂的研究，导致了两种子囊壳形成菌株的发现。为了评估 Phoma clematidina 的多样性并阐明其有性型-无性型关系，对 18 株 P. clematidina 菌株、代表 Didymellaceae 中 Phoma 节的参考菌株以及与 Clematis 相关的相关物种的菌株进行了系统发育分析。使用 ITS1、ITS2 和 5.8S rRNA 基因、ss-微管蛋白基因和 28S rRNA 基因的部分序列来阐明种内和种间关系。这些分析表明，P. clematidina 分为三个支持良好的分支，似乎与宿主特异性的差异有关。基于这些发现，新描述了 Didymella clematidis，并修订了 P. clematidina 和 D. vitalbina 的描述。
