School of Materials Science and Engineering, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, West Xianning Road, Xi'an 710049, People's Republic of China.
J Biomed Mater Res A. 2010 Aug;94(2):450-6. doi: 10.1002/jbm.a.32655.
The biomedical properties of novel biodegradable copoly(amino acid)s based on 6-aminocaproic acid and L-proline were analyzed in this article. The cytotoxicity of the copolymer films was tested in vitro using human embryonic kidney (HEK) 293 cells. The cell proliferation, cell cycle, cell apoptosis, and hemolysis of the polymers were also investigated. No significant cytotoxic response was detected statistically by cytotoxicity assay, and the results of cell apoptosis and cell cycle showed that there were no statistically significant differences in them. Generally, the cells spread and grew well on polymer film. Meanwhile, the extent of hemolysis on the polymers was acceptable. Evaluation of cytotoxicity by cell cycle and apoptosis as a supplementary assay is correspondingly discussed in this article.
本文分析了基于 6-氨基己酸和 L-脯氨酸的新型可生物降解共聚（氨基酸）的生物医学特性。使用人胚肾（HEK）293 细胞在体外测试了共聚物薄膜的细胞毒性。还研究了聚合物的细胞增殖、细胞周期、细胞凋亡和溶血。细胞毒性测定未检测到统计学上显著的细胞毒性反应，细胞凋亡和细胞周期的结果表明它们之间没有统计学上的显著差异。通常，细胞在聚合物薄膜上良好地扩散和生长。同时，聚合物的溶血程度是可以接受的。本文还相应地讨论了细胞周期和凋亡作为补充测定法评估细胞毒性的问题。
