School of Physical Science and Technology, Sichuan University, Chengdu, People's Republic of China.
Int J Nanomedicine. 2012;7:1287-95. doi: 10.2147/IJN.S28978. Epub 2012 Mar 8.
A nano calcium-deficient hydroxyapatite (n-CDHA)-multi(amino acid) copolymer (MAC) composite bone substitute biomaterial was prepared using an in situ polymerization method. The composition, structure, and compressive strength of the composite was characterized, and the in vitro degradability in phosphate-buffered solution and preliminary cell responses to the composite were investigated.
The composite comprised n-CDHA and an amide linkage copolymer. The compressive strength of the composite was in the range of 88-129 MPa, varying with the amount of n-CDHA in the MAC (ranging from 10 wt% to 50 wt%). Weight loss from the composite increased (from 32.2 wt% to 44.3 wt%) with increasing n-CDHA content (from 10 wt% to 40 wt%) in the MAC after the composite was soaked in phosphate-buffered solution for 12 weeks. The pH of the soaking medium varied from 6.9 to 7.5. MG-63 cells with an osteogenic phenotype were well adhered and spread on the composite surface. Viability and differentiation increased with time, indicating that the composite had no negative effects on MG-63 cells.
The n-CDHA-MAC composite had good cytocompatibility and has potential to be used as a bone substitute.
采用原位聚合法制备了纳米缺钙羟基磷灰石(n-CDHA)-多(氨基酸)共聚物(MAC)复合骨替代生物材料。对复合材料的组成、结构和抗压强度进行了表征，并研究了其在磷酸盐缓冲溶液中的体外降解性和对细胞的初步反应。
复合材料由 n-CDHA 和酰胺键共聚物组成。复合材料的抗压强度在 88-129 MPa 范围内，随 MAC 中 n-CDHA 的含量（10wt%-50wt%）而变化。在磷酸盐缓冲溶液中浸泡 12 周后，复合材料中 n-CDHA 含量（10wt%-40wt%）增加，复合材料的失重（从 32.2wt%增加到 44.3wt%）增加。浸泡介质的 pH 值从 6.9 变化到 7.5。具有成骨表型的 MG-63 细胞在复合材料表面很好地黏附和展开。细胞活力和分化随时间增加，表明复合材料对 MG-63 细胞没有不良影响。
n-CDHA-MAC 复合材料具有良好的细胞相容性，有望用作骨替代物。
