Akush Ginekol (Sofiia). 2009;48(3):19-26.
Our study included material of 30 patients with ovarian polycystosis (PCOS-polycystic ovary syndrome). Informed consent was obtained from patients before their laparoscopic surgery and biopsy. A total of 16 oocytes and small follicles were isolated from (33.33%) of cases by stereomicroscopic dissection. This was followed by follicle cells removing, zona pellucida dissolving, hypotonic incubation and fixation onto slides. On the fixed samples, FISH with markers for chromosomes 18, 21 and X was performed. Analysis was successful in 11 (68.75%) of objects (5 follicles and 6 oocytes). Five oocytes (31.25%) had a negative FISH reaction because of condensed chromatin. This indicated cellular ageing which is characteristic of atretic follicles.
(1) We obtained data about chromatin and chromosomal status of analysed oocytes, which correlated with ovarian reserve dynamics in PCOS and the processes of maturation and chromatin ageing in growing and atretic follicles. (2) The study has provided information about biological parameters of ovarian tissue in PCO that could be useful for obtaining precise patients' diagnosis and for their reproductive prognosis. (3) The problem needs more extensive studies because the number of analyzed oocytes was insufficient to allow data comparison for aneuploidy in PCOS ovaries vs. normally functioning ovaries.
我们的研究纳入了30例多囊卵巢综合征（PCOS）患者的材料。在患者进行腹腔镜手术和活检前已获得知情同意。通过体视显微镜解剖从（33.33%）的病例中总共分离出16个卵母细胞和小卵泡。随后进行卵泡细胞去除、透明带溶解、低渗孵育并固定于载玻片上。在固定样本上，进行了针对18号、21号染色体和X染色体的荧光原位杂交（FISH）。11个样本（5个卵泡和6个卵母细胞，占68.75%）分析成功。5个卵母细胞（占31.25%）因染色质浓缩而出现FISH阴性反应。这表明细胞老化，这是闭锁卵泡的特征。
（1）我们获得了有关所分析卵母细胞染色质和染色体状态的数据，这些数据与PCOS患者的卵巢储备动态以及生长中和闭锁卵泡的成熟和染色质老化过程相关。（2）该研究提供了有关PCO中卵巢组织生物学参数的信息，这对于准确诊断患者及其生殖预后可能有用。（3）由于所分析的卵母细胞数量不足以对PCOS卵巢与正常功能卵巢的非整倍体数据进行比较，该问题需要更广泛的研究。
