Akutsu Tomoko, Ikegaya Hiroshi, Watanabe Ken, Fukushima Hisayo, Motani Hisako, Iwase Hirotaro, Sakurada Koichi
National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
J Forensic Sci. 2010 May;55(3):742-6. doi: 10.1111/j.1556-4029.2010.01325.x. Epub 2010 Feb 25.
In this study, Tamm-Horsfall protein (THP), a major component of urinary protein, and uroplakin III (UPIII), a transmembrane protein widely regarded as a urothelium-specific marker, were evaluated for forensic identification of urine by ELISA and/or immunohistochemistry. THP was detected in urine, but not in plasma, saliva, semen, vaginal fluid, or sweat by the simple ELISA method developed in this study. In addition, most aged urine stains showed positive results. The urine specificity of THP was confirmed by gene expression analysis. Therefore, as reported previously, ELISA detection of THP can be used as a presumptive test for urine identification. UPIII was specific for immunohistochemical staining of cells in centrifuged precipitate of urine. However, ELISA and RT-PCR for UPIII were not specific for urine. UPIII may be applicable for forensic urine identification by immunohistochemistry.
在本研究中，通过酶联免疫吸附测定（ELISA）和/或免疫组织化学法，对尿蛋白的主要成分Tamm-Horsfall蛋白（THP）以及被广泛视为尿路上皮特异性标志物的跨膜蛋白uroplakin III（UPIII）进行评估，以用于尿液的法医鉴定。通过本研究开发的简单ELISA方法，在尿液中检测到了THP，但在血浆、唾液、精液、阴道分泌物或汗液中未检测到。此外，大多数陈旧尿渍显示出阳性结果。通过基因表达分析证实了THP的尿液特异性。因此，如先前报道，ELISA检测THP可作为尿液鉴定的初步试验。UPIII对尿液离心沉淀物中的细胞进行免疫组织化学染色具有特异性。然而，针对UPIII的ELISA和逆转录聚合酶链反应（RT-PCR）对尿液并不具有特异性。UPIII可能适用于通过免疫组织化学进行法医尿液鉴定。
