Park Byung-Soon, Kim Won-Serk, Choi Joon-Seok, Kim Hyung-Ki, Won Jong-Hyun, Ohkubo Fumio, Fukuoka Hirotaro
Leaders Clinic, Seoul, Korea.
Biomed Res. 2010 Feb;31(1):27-34. doi: 10.2220/biomedres.31.27.
Adipose-derived stem cells (ADSCs) and their secretomes mediate diverse skin-regeneration effects, such as wound-healing and antioxidant protection, that are enhanced by hypoxia. We investigated the hair-growth-promoting effect of conditioned medium (CM) of ADSCs to determine if ADSCs and their secretomes regenerate hair and if hypoxia enhances hair regeneration. If so, we wanted to identify the factors responsible for hypoxia-enhanced hair-regeneration. We found that ADSC-CM administrated subcutaneously induced the anagen phase and increased hair regeneration in C(3)H/NeH mice. In addition, ADSC-CM increased the proliferation of human follicle dermal papilla cells (HFDPCs) and human epithelial keratinocytes (HEKs), which are derived from two major cell types present in hair follicles. We investigated the effect of hypoxia on ADSC function using the same animal model in which hypoxia increased hair regrowth. Forty-one growth factors in ADSC-CM from cells cultured under hypoxic or normoxic conditions were analyzed. The secretion of insulin-like growth factor binding protein (IGFBP)-1, IGFBP-2, macrophage colony-stimulating factor (M-CSF), M-CSF receptor, platelet-derived growth factor receptor-beta, and vascular endothelial growth factor was significantly increased by hypoxia, while the secretion of epithelial growth factor production was decreased. It is reasonable to conclude that ADSCs promote hair growth via a paracrine mechanism that is enhanced by hypoxia.
脂肪来源干细胞（ADSCs）及其分泌组介导多种皮肤再生效应，如伤口愈合和抗氧化保护，缺氧可增强这些效应。我们研究了ADSCs条件培养基（CM）促进毛发生长的作用，以确定ADSCs及其分泌组是否能再生毛发，以及缺氧是否能增强毛发再生。如果是这样，我们想确定导致缺氧增强毛发再生的因素。我们发现，皮下注射ADSC-CM可诱导C(3)H/NeH小鼠进入生长期并增加毛发再生。此外，ADSC-CM可增加人毛囊真皮乳头细胞（HFDPCs）和人上皮角质形成细胞（HEKs）的增殖，这两种细胞是毛囊中的两种主要细胞类型。我们使用相同的动物模型研究了缺氧对ADSC功能的影响，在该模型中缺氧可增加毛发再生。分析了在缺氧或常氧条件下培养的细胞的ADSC-CM中的41种生长因子。缺氧显著增加了胰岛素样生长因子结合蛋白（IGFBP）-1、IGFBP-2、巨噬细胞集落刺激因子（M-CSF）、M-CSF受体、血小板衍生生长因子受体-β和血管内皮生长因子的分泌，而上皮生长因子的分泌则减少。有理由得出结论，ADSCs通过一种旁分泌机制促进毛发生长，缺氧可增强这种机制。
