Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
Nat Protoc. 2010 Mar;5(3):479-90. doi: 10.1038/nprot.2009.233. Epub 2010 Feb 18.
Marine-derived fungi have been shown in recent years to produce a plethora of new bioactive secondary metabolites, some of them featuring new carbon frameworks hitherto unprecedented in nature. These compounds are of interest as new lead structures for medicine as well as for plant protection. The aim of this protocol is to give a detailed description of methods useful for the isolation and cultivation of fungi associated with various marine organisms (sponges, algae and mangrove plants) for the extraction, characterization and structure elucidation of biologically active secondary metabolites produced by these marine-derived endophytic fungi, and for the preliminary evaluation of their pharmacological properties based on rapid 'in house' screening systems. Some results exemplifying the positive outcomes of the protocol are given at the end. From sampling in marine environment to completion of the structure elucidation and bioactivity screening, a period of at least 3 months has to be scheduled.
近年来的研究表明，海洋来源的真菌能够产生大量新型生物活性次生代谢产物，其中一些化合物具有全新的碳骨架，在自然界中前所未见。这些化合物作为新型药物先导结构以及植物保护剂具有重要的研究意义。本方案详细介绍了从各种海洋生物（海绵、藻类和红树林植物）中分离和培养相关真菌的方法，包括提取、鉴定和结构解析这些海洋内生真菌产生的具有生物活性的次生代谢产物，以及基于快速“内部”筛选系统对其药理性质进行初步评价。文末给出了一些方案实施的结果示例。从海洋环境采样到完成结构解析和生物活性筛选，至少需要 3 个月的时间。
