Ahlenius Henrik, Kokaia Zaal
Section of Restorative Neurology, Laboratory of Neural Stem Cell Biology, Lund Stem Cell Center, Lund, Sweden.
Methods Mol Biol. 2010;633:241-52. doi: 10.1007/978-1-59745-019-5_18.
Neural stem cells are defined as cells that either gives rise to or derives from the cells of the central nervous system and have the unique properties of stem cells, i.e. self-renewal and multipotentiality. One of the widely used methods of expanding neural stem cells under culture conditions is based on the capacity of these cells to divide continuously when cultured in serum-free medium supplemented with various growth factors. One common method used is to grow neural stem cells as free-floating aggregates of cells called neurospheres. Neurospheres can be generated from several structures of the embryonic and adult mammalian brain. Although viable lines can be generated from crude extracts of brain, a precise dissection is crucial to get a pure population of cells. Here we describe methods for dissection, isolation and generation of neurospheres from embryonic ganglionic eminences and adult subventricular zone of mice and rats.
神经干细胞被定义为能够产生中枢神经系统细胞或从中枢神经系统细胞衍生而来的细胞，并且具有干细胞的独特特性，即自我更新和多能性。在培养条件下扩增神经干细胞的一种广泛使用的方法是基于这些细胞在补充有各种生长因子的无血清培养基中培养时连续分裂的能力。一种常用的方法是将神经干细胞培养成称为神经球的细胞自由漂浮聚集体。神经球可以从胚胎和成年哺乳动物大脑的几种结构中产生。虽然可以从脑的粗提物中产生有活力的细胞系，但精确解剖对于获得纯细胞群体至关重要。在这里，我们描述了从小鼠和大鼠的胚胎神经节隆起和成年脑室下区进行解剖、分离和生成神经球的方法。
