College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Biochem Biophys Res Commun. 2010 Apr 2;394(2):354-9. doi: 10.1016/j.bbrc.2010.03.011. Epub 2010 Mar 4.
In present research, the full-length cDNA and the genomic sequence of a novel cold-regulated gene, CsCOR1, were isolated from Camellia sinensis L. The deduced protein CsCOR1 contains a hydrophobic N-terminus as a signal peptide and a hydrophilic C-terminal domain that is rich in glycine, arginine and proline. Two internal repetitive tridecapeptide fragments (HSVTAGRGGYNRG) exist in the middle of the C-terminal domain and the two nucleotide sequences encoding them are identical. CsCOR1 was localized in the cell walls of transgenic-tobaccos via CsCOR1::GFP fusion approach. The expression of CsCOR1 in tea leaves was enhanced dramatically by both cold- and dehydration-stress. And overexpression of CsCOR1 in transgenic-tobaccos improved obviously the tolerance to salinity and dehydration.
在本研究中，从茶树中分离出一个新的冷调节基因 CsCOR1 的全长 cDNA 和基因组序列。推测的蛋白 CsCOR1 含有一个疏水性的 N 端作为信号肽和一个富含甘氨酸、精氨酸和脯氨酸的亲水性 C 端结构域。C 端结构域的中间存在两个内部重复的十三肽片段（HSVTAGRGGYNRG），并且编码它们的两个核苷酸序列相同。通过 CsCOR1::GFP 融合方法，CsCOR1 定位于转基因烟草的细胞壁中。CsCOR1 在茶树叶片中的表达在冷胁迫和脱水胁迫下显著增强。并且，在转基因烟草中超表达 CsCOR1 明显提高了耐盐性和耐旱性。
