Hu Zhe, Ban Qiuyan, Hao Jing, Zhu Xiangxiang, Cheng Yaohua, Mao Junlin, Lin Mengling, Xia Enhua, Li Yeyun
State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects, Anhui Agricultural University, Hefei, China.
Front Plant Sci. 2020 Jul 23;11:921. doi: 10.3389/fpls.2020.00921. eCollection 2020.
C-repeat (CRT)/dehydration responsive element (DRE)-binding factor CBFs, a small family of genes encoding transcriptional activators, play important roles in plant cold tolerance. In this study, a comprehensive genome-wide analysis was carried out to identify and characterize the functional dynamics of in tea plant (). A total of 6 genes were obtained from the tea plant genome and named . All of the had an AP2/ERF DNA-binding domain and nuclear localization signal (NLS) sequence. CsCBF-eGFP fusion and DAPI staining analysis confirmed the nuclear localization of the CsCBFs. Transactivation assays showed that the CsCBFs, except CsCBF1, had transcriptional activity. CsCBF expression was differentially induced by cold, heat, PEG, salinity, ABA, GA, MeJA, and SA stresses. In particular, the genes were significantly induced by cold treatments. To further characterize the functions of genes, we overexpressed the gene in plants. The resulting transgenic plants showed increased cold tolerance compared with the wild-type plant. The enhanced cold tolerance of the transgenic plants was potentially achieved through an ABA-independent pathway. This study will help to increase our understanding of genes and their contributions to stress tolerance in tea plants.
C-重复序列（CRT）/脱水响应元件（DRE）结合因子CBFs是一个编码转录激活因子的小基因家族，在植物抗寒中发挥重要作用。在本研究中，进行了全面的全基因组分析，以鉴定和表征茶树（）中基因的功能动态。从茶树基因组中总共获得了6个基因，并命名为。所有这些基因都具有AP2/ERF DNA结合结构域和核定位信号（NLS）序列。CsCBF-eGFP融合和DAPI染色分析证实了CsCBFs的核定位。转录激活分析表明，除CsCBF1外，CsCBFs具有转录活性。CsCBF的表达受到冷、热、PEG、盐度、ABA、GA、MeJA和SA胁迫的差异诱导。特别是，这些基因在冷处理下被显著诱导。为了进一步表征基因的功能，我们在植物中过表达了基因。与野生型植物相比，所得转基因植物表现出增强的抗寒性。转基因植物抗寒性的增强可能是通过一条不依赖ABA的途径实现的。本研究将有助于增进我们对基因及其对茶树胁迫耐受性贡献的理解。
